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Analysis on improvement in resolution by excitation beam modulation in stimulated emission depletion nanoscopy

  • Special Section: Regular Paper
  • The 11th International Conference on Optics-Photonics Design & Fabrication (ODF’18), Hiroshima, Japan
  • Published:
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Abstract

CW STED nanoscopy using illumination of continuous wave is considerably easy and less expensive to construct compared with the pulsed STED nanoscopy. In this study, to improve the resolution of CW STED nanoscopy, we analyzed the imaging characteristics of CW STED nanoscopy by amplitude modulation of incident light flux of the excitation beam illumination considering the polarization state and geometry of the pupil mask for amplitude modulation. We analyzed the imaging characteristics of STED nanoscopy by applying the characteristic, which shows an extremely confined electric field in transverse direction when the light waves with high spatial frequencies and with the same polarization direction are diffracted and interfered in the focal region. By applying linearly polarized illumination and the mixed-shaped aperture composed of the bow tie-shaped blocking area and the circular blocking aperture area, we analyzed that imaging resolution can be enhanced above 20% higher than the resolution of the conventional CW STED nanoscopy.

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Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government(MSIT) (2015R1A5A1037668, NRF-2019R1C1C1010911).

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Authors and Affiliations

  1. School of Mechanical Engineering, Yonsei University, 50 Yonsei-ro, Sudaemungu, Seoul, 120-749, Korea

    Geon Lim & No-Cheol Park

  2. Department of Smart Manufacturing Applied Engineering, Hanbat National University, 125, Dongseo-daero, Yuseong-gu, Daejeon, 34158, Korea

    Wan-Chin Kim

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  1. Geon Lim
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  2. No-Cheol Park
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Correspondence to No-Cheol Park or Wan-Chin Kim.

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Lim, G., Park, NC. & Kim, WC. Analysis on improvement in resolution by excitation beam modulation in stimulated emission depletion nanoscopy. Opt Rev 26, 512–521 (2019). https://doi.org/10.1007/s10043-019-00531-5

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