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Practical technique to quantify small, dense low-density lipoprotein cholesterol using dynamic light scattering

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Abstract

Quantification of small, dense low-density lipoprotein (sdLDL) cholesterol is clinically significant. We propose a practical technique to estimate the amount of sdLDL cholesterol using dynamic light scattering (DLS). An analytical solution in a closed form has newly been obtained to estimate the weight fraction of one species of scatterers in the DLS measurement of two species of scatterers. Using this solution, we can quantify the sdLDL cholesterol amount from the amounts of the low-density lipoprotein cholesterol and the high-density lipoprotein (HDL) cholesterol, which are commonly obtained through clinical tests. The accuracy of the proposed technique was confirmed experimentally using latex spheres with known size distributions. The applicability of the proposed technique was examined using samples of human blood serum. The possibility of estimating the sdLDL amount using the HDL data was demonstrated. These results suggest that the quantitative estimation of sdLDL amounts using DLS is feasible for point-of-care testing in clinical practice.

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Acknowledgments

The authors would like to thank Professor Masahiro Tsuji of Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido for his valuable advice related to this study. This work was financially supported by the Thailand Research Fund, the Commission on Higher Education and Ubon Ratchathani University (TRF-CHE-UBU grant number MRG5580083). Part of this research was also supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Faculty of Engineering, Ubon Ratchathani University, 85 Sathonlamark Rd., Warinchamrab, Ubon Ratchathani, 34190, Thailand

    Suchin Trirongjitmoah

  2. Graduate School of Information Science and Technology, Hokkaido University, N14, W9, Kita-ku, Sapporo, 060-0814, Japan

    Kazuya Iinaga

  3. Faculty of Health Sciences, Hokkaido University, N12, W5, Kita-ku, Sapporo, 060-0812, Japan

    Toshihiro Sakurai & Hitoshi Chiba

  4. Faculty of Engineering, Chulalongkorn University, Phayathai, Pathumwan, Bangkok, 12120, Thailand

    Mana Sriyudthsak

  5. Graduate School of Information, Production and Systems, Waseda University, 2-7 Hibikino, Wakamatsu-ku, Kitakyushu, 808-0135, Japan

    Koichi Shimizu

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  1. Suchin Trirongjitmoah
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  2. Kazuya Iinaga
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  3. Toshihiro Sakurai
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  5. Mana Sriyudthsak
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Correspondence to Suchin Trirongjitmoah.

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Informed consent was obtained from all the individuals participating in this study. This study was approved by the ethics review board of the Faculty of Health Sciences, Hokkaido University, Japan (approval number 09-38).

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Trirongjitmoah, S., Iinaga, K., Sakurai, T. et al. Practical technique to quantify small, dense low-density lipoprotein cholesterol using dynamic light scattering. Opt Rev 23, 265–272 (2016). https://doi.org/10.1007/s10043-016-0187-9

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  • DOI: https://doi.org/10.1007/s10043-016-0187-9

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