Abstract
We propose a novel full-electronically controlled laser confocal microscope in which a liquid-crystal-on-silicon spatial light modulator and a custom CMOS imaging sensor are synchronized for performing multi-beam confocal imaging. Adaptive wavefront modulation for functional multi-beam excitation can be achieved by displaying appropriate computer generated holograms on the spatial light modulator, in consideration of the numerical aperture of the focusing objective. We also adopted a custom CMOS imaging sensor to realize multi-beam confocal microscopy without any physical pinhole. The confocality of this microscope was verified by improvements in transverse and axial resolutions of fluorescent micro-beads.
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Acknowledgments
The authors are grateful to A. Hiruma (President), T. Hara (Director) and H. Toyoda of Hamamatsu Photonics for their numerous encouragements throughout this work. We also thank T. Otsu-Hyodo, and K. Watanabe for their helpful support in the experiments, and Y. Ohtake and T. Ando for valuable discussions. This work is partially supported by Development of Systems and Technology for Advanced Measurement and Analysis, Japan Science and Technology Agency.
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Takiguchi, Y., Seo, MW., Kagawa, K. et al. Mechanical scanner-less multi-beam confocal microscope with wavefront modulation. Opt Rev 23, 364–368 (2016). https://doi.org/10.1007/s10043-015-0159-5
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DOI: https://doi.org/10.1007/s10043-015-0159-5