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A plastic scintillator film for an electron beam-excitation assisted optical microscope

  • Special Section: Regular Paper
  • Biomedical Imaging and Sensing Conference (BISC’14), Yokohama, Japan
  • Published:
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Abstract

A plastic scintillator film for use in an electron beam excitation-assisted (EXA) optical microscope is characterized. The thin film scatters an incident electron beam weakly and generates high intensity nanoscale luminescence excited by the beam spot. For high spatial resolution and signal to noise, an EXA microscope requires a thin high-efficiency scintillator film. Homogeneous plastic scintillators with thicknesses ranging from 60 to 2800 nm were fabricated on silicon nitride via spin coating. The emission intensity was examined as a function of film thickness and the accelerating voltage of the incident electron beam. The emission wavelength can be tuned by changing scintillator materials in the film matrix. To demonstrate a plastic scintillator film performance with an EXA microscope, time-lapse images of yeast cells were acquired.

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References

  1. S.W. Hell, J. Wichmann, Optics Lett. 19, 780 (1994)

    Article  ADS  Google Scholar 

  2. R. Heintzmann, T.M. Jovin, C. Cremer, J. Opt. Soc. Am. A 19, 1599 (2002)

    Article  ADS  Google Scholar 

  3. M.G.L. Gustafsson, J. Proc. Natl. Acad. Sci. 102, 13081 (2005)

    Article  ADS  Google Scholar 

  4. K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, S. Kawata, Phys. Rev. Lett. 99, 228105 (2007)

    Article  ADS  Google Scholar 

  5. E. Betzig, G.H. Patterson, R. Sougrat, O.W. Lindwasser, S. Olenych, J.S. Bonifacino, M.W. Davidson, J. Lippincott-Schwartz, H.F. Hess, Science 313, 1642 (2006)

    Article  ADS  Google Scholar 

  6. M.J. Rust, M. Bates, X. Zhuang, Nat. Methods 3, 793 (2006)

    Article  Google Scholar 

  7. E.H. Synge, Philos. Mag. 6, 356 (1928)

    Article  Google Scholar 

  8. E.A. Ash, G. Nicholls, Appl. Phys. Lett. 44, 651 (1984)

    Article  Google Scholar 

  9. D. Pohl, W. Denk, M. Lanz, Appl. Phys. Lett. 44, 651 (1984)

    Article  ADS  Google Scholar 

  10. E. Betzig, M. Isaacson, A. Lewis, Appl. Phys. Lett. 51, 2088 (1987)

    Article  ADS  Google Scholar 

  11. W. Inami, K. Nakajima, A. Miyakawa, Y. Kawata, Opt. Express 18, 12897 (2010)

    Article  ADS  Google Scholar 

  12. A. Sugita, M. Kamiya, C. Morita, A. Miyake, Y. Nawa, Y. Masuda, W. Inami, H. Kominami, Y. Nakanishi, Y. Kawata, Opt. Mater. Express 4, 155 (2013)

    Article  Google Scholar 

  13. A. Miyake, S. Kanamori, Y. Nawa, W. Inami, H. Kominami, Y. Kawata, Y. Nakanishi: Jpn. J. Appl. Phys. 53, 04EH11 (2014)

  14. W. Inami, J. Fujiwara, M. Fukuta, A. Ono, Y. Kawata, Appl. Phys. Lett. 101, 151104 (2012)

    Article  ADS  Google Scholar 

  15. D.C. Joy, Monte Carlo modeling for electron microscopy and microanalsysis (Oxford University Press, New York, 1995)

    Google Scholar 

  16. D. Drouin, A.R. Couture, D. Joly, X. Tastet, V. Aimez, R. Gauvin, Scanning 29, 92 (2007)

    Article  Google Scholar 

  17. K.S. Yee, IEEE Trans. Antennas Propagat. 14, 302 (1966)

    Article  ADS  MATH  Google Scholar 

Download references

Acknowledgments

This work was supported by CREST of JST (Japan Science and Technology Agency).

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Authors and Affiliations

  1. Faculty of Engineering, Shizuoka University, 3-5-1 Johoku, Nake-ku, Hamamatsu, Shizuoka, 432-8561, Japan

    Wataru Inami, Masahiro Fukuta, Yuriko Masuda & Yasunori Nawa

  2. CREST, Japan Science and Technology Agency, 7 Gobancho, Chiyoda-ku, Tokyo, 102-0075, Japan

    Wataru Inami, Atsushi Ono, Sheng Lin & Yoshimasa Kawata

  3. Research Institute of Electronics, Shizuoka University, Johoku, Naka, Hamamatsu, 432-8011, Japan

    Atsushi Ono, Yoshimasa Kawata & Susumu Terakawa

  4. Photon Medical Research Center, Hamamatsu University School of Medicine, Handayama, Higashi, Hamamatsu, 431-3192, Japan

    Susumu Terakawa

Authors
  1. Wataru Inami
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  2. Masahiro Fukuta
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  3. Yuriko Masuda
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  4. Yasunori Nawa
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  5. Atsushi Ono
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  6. Sheng Lin
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  7. Yoshimasa Kawata
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  8. Susumu Terakawa
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Correspondence to Wataru Inami.

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Inami, W., Fukuta, M., Masuda, Y. et al. A plastic scintillator film for an electron beam-excitation assisted optical microscope. Opt Rev 22, 354–358 (2015). https://doi.org/10.1007/s10043-015-0043-3

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  • DOI: https://doi.org/10.1007/s10043-015-0043-3

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