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Quantitative Evaluation of Oxygenation and Metabolism in the Human Skeletal Muscle

  • ENVIRONMENTAL, BIOLOGICAL, AND SPACE OPTICS
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Abstract

The forearm muscles of five healthy males were monitored for changes in microvessel hemoglobin saturation (SO2-TRS) by near infrared time-resolved spectroscopy (NIRTRS) and changes in phosphorus metabolites by magnetic resonance spectroscopy (31P-MRS) during 12 min of resting arterial occlusion. Muscle oxygenation and phosphorus metabolites were also measured during grip exercises at varying intensities. Upon the initiation of occlusion, SO2-TRS fell progressively until it reached a plateau in the latter half of the occlusion. Phosphocreatine (PCr) began to decrease around 6 min after the initiation of arterial occlusion. The resting O2 store and O2 consumption were 295 μM and 0.95 μM/sec, respectively-values which reasonably agree with the reported results. A significant correlation was observed between the changes in SO2-TRS and PCr during exercise (r2 = 0.80, p < 0.001). These results indicate that NIRTRS is able to provide reliable information about resting metabolism and oxidative rate during exercise. NIRTRS and MRS are useful to monitor oxygenation and energetics noninvasively in the human muscle.

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References

  1. B. Chance, T. M. Dait, C. Zhang, T. Hamaoka and F. Hagerman: Am. J. Physiol. 262 (1992) C766.

    PubMed  Google Scholar 

  2. T. Hamaoka, H. Iwane, T. Shimomitsu, T. Katsumura, N. Murase, S. Nishio, T. Osada, Y. Kurosawa and B. Chance: J. Appl. Physiol. 81 (1996) 1410.

    PubMed  Google Scholar 

  3. N. B. Hampson and C. A. Piantadosi: J. Appl. Physiol. 64 (1988) 2449.

    PubMed  Google Scholar 

  4. B. Chance, S. Nioka, J. Kent-Braun, K. K. McCully, M. Fountain, R. Greenfeld and G. Holtom: Anal. Biochem. 174 (1988) 698.

    Article  PubMed  Google Scholar 

  5. J. Hansen, G. D. Thomas, S. A. Harris, W. J. Parsons and R. G. Victor: J. Clin. Invest. 98 (1996) 584.

    PubMed  Google Scholar 

  6. F. F. Jobsis: Science 198 (1977) 1264.

    PubMed  Google Scholar 

  7. H. Higuchi, T. Hamaoka, T. Takayuki, S. Nishio, R. Kime, M. Murakami and T. Katsumura: Eur. J. Appl. Physiol. 87 (2002) 343.

    Article  PubMed  Google Scholar 

  8. V. Quaresima, S. Homma, K. Azuma, S. Shimizu, F. Chiarotti, M. Ferrari and A. Kagaya: Eur. J. Appl. Physiol. 84 (2001) 387.

    Article  PubMed  Google Scholar 

  9. M. A. Franceschini, D. A. Boas, A. Zourabian, S. G. Diamond, S. Nadgir, D. W. Lin, J. B. Moore and S. Fantini: J. Appl. Physiol. 92 (2002) 372.

    PubMed  Google Scholar 

  10. T. Komiyama, H. Shigematsu, H. Yasuhara and T. Muto: Eur. J. Vasc. Surg. 8 (1994) 294.

    Article  PubMed  Google Scholar 

  11. J. R. Wilson, D. Mancini, K. K. McCully, N. Ferraro, V. Lanoce and B. Chance: Circulation 80 (1989) 1668.

    PubMed  Google Scholar 

  12. D. T. Delpy, M. Cope, P. P. van der Zee, S. Arridge, S. Wrary and J. Wyatt: Phys. Med. Biol. 33 (1998) 1422.

    Google Scholar 

  13. M. Ferrari, Q. Wei, L. Carraresi, R. De Blasi and G. Zaccanti: J. Photochem. Photobiol. 16 (1992) 141.

    Article  Google Scholar 

  14. T. Hamaoka, T. Katsumura, N. Murase, S. Nishio, T. Osada, T. Sako, H. Higuchi, Y. Kurosawa, T. Shimomitsu, M. Miwa and B. Chance: J. Biomed. Opt. 5 (2000) 102.

    Article  PubMed  Google Scholar 

  15. E. M. Sevick, B. Chance, J. Leigh, S. Nioka and M. Maris: Anal. Biochem. 195 (1991) 330.

    Article  PubMed  Google Scholar 

  16. B. Beauvoit, S. M. Evans, T. W. Jenkins, E. M. Miller and B. Chance: Anal. Biochem. 226 (1995) 167.

    Article  PubMed  Google Scholar 

  17. R. A. De Blasi, M. Cope and M. Ferrari: Adv. Exp. Med. Biol. 317 (1992) 771.

    PubMed  Google Scholar 

  18. M. S. Patterson, B. Chance and B. C. Wilson: J. Appl. Optics 28 (1989) 2331.

    Google Scholar 

  19. R. Harris, E. Hultman and L.-O. Nordesjo: Scand. J. Clin. and Lab. Invest. 33 (1974) 109.

    Google Scholar 

  20. M. J. Kushmerick and R. A. Meyer: Am. J. Physiol. 248 (1985) C542.

    PubMed  Google Scholar 

  21. J. Bosman, G. Tangelder, M. Oude Egbrink, R. Reneman and D. Slaaf: Am. J. Physiol. 269 (1995) H1048.

    PubMed  Google Scholar 

  22. R. A. Meyer: Am. J. Physiol. 254 (1998) C548.

    Google Scholar 

  23. K. K. McCully, S. Iotti, K. Kendrick, Z. Wang, J. D. Posner, J. S. Leigh and B. Chance: J. Appl. Physiol. 77 (1994) 5.

    PubMed  Google Scholar 

  24. T. K. Tran, N. Sailasuta, U. Kreutzer, R. Hurd, Y. Chung, P. Mole, S. Kuno and T. Jue: Am. J. Physiol. 276 (1999) R1682.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Preventive Medicine and Public Health, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo, Japan

    Takafumi Hamaoka, Takuya Osada, Norio Murase, Hiroyuki Higuchi, Yuko Kurosawa & Toshihito Katsumura

  2. National Institute of Fitness and Sports, 1 Shiromizu-cho, Kanoya, Kagoshima, Japan

    Takafumi Hamaoka

  3. Exercise Physiology, Department of Food & Nutrition, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, Japan

    Takayuki Sako

  4. Hamamatsu Photonics, 5000 Hiraguchi, Hamakita City, Shizuoka Prefecture, Japan

    Mitsuharu Miwa

  5. Department of Biochemistry and Biophysics, University of Pennsylvania, D501 Richards Building, Philadelphia, USA

    Britton Chance

Authors
  1. Takafumi Hamaoka
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  2. Takuya Osada
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  3. Norio Murase
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  4. Takayuki Sako
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  5. Hiroyuki Higuchi
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  6. Yuko Kurosawa
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  7. Mitsuharu Miwa
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  8. Toshihito Katsumura
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  9. Britton Chance
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Correspondence to Takafumi Hamaoka.

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Hamaoka, T., Osada, T., Murase, N. et al. Quantitative Evaluation of Oxygenation and Metabolism in the Human Skeletal Muscle. OPT REV 10, 493–497 (2003). https://doi.org/10.1007/s10043-003-0493-x

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  • DOI: https://doi.org/10.1007/s10043-003-0493-x

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