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Bacterial Etiology for Chronic Villitis Is Not Supported by Polymerase Chain Reaction for 16S rRNA DNA

Pediatric and Developmental Pathology

Abstract

Chronic villitis is characterized by chorionic villi infiltrated by lymphocytes, histiocytes, and sometimes plasma cells. In a small percentage of cases, an infectious agent can be demonstrated within areas of chronic villitis. However, the pathogenesis of most lesions is idiopathic. Chronic villitis may represent the direct spread of chronic endometrial infection by bacterial organisms that are particularly problematic for culture. To test this hypothesis, polymerase chain reaction (PCR) using primers for the universal bacterial 16S rRNA DNA was performed on DNA extracted from areas of chronic villitis selected from placentas in the Yale Pathology database. Specific areas of chronic villitis were first confirmed by examination of sections stained with hematoxylin and eosin and then removed from archived paraffin blocks. Control tissue spiked with known bacterial counts was also prepared to test the sensitivity of the experiment. All tissue was deparaffinized, dehydrated, and digested with proteinase K. DNA extraction was performed with the Gentra Puregene kit. PCR was done using primers p11 and p13 for the 16S rRNA DNA. The 233-bp amplified target product was identified by agarose gel electrophoresis. Nineteen specimens with multifocal chronic villitis without confinement to anchoring villi were studied. None of the chronic villitis specimens had a demonstrable product using the PCR primers for 16S rRNA DNA, despite adequate DNA in the samples and controls. The assay was sensitive down to approximately 1500 bacteria per specimen. In conclusion, these data do not support a bacterial etiology for chronic villitis.

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References

  1. Knox WF, Fox H. Villitis of unknown aetiology: its incidence and significance in placentae from a British population Placenta 1984;5:395–402

    PubMed  CAS  Google Scholar 

  2. Labarrere C, et al. Chronic villitis of unknown aetiology in placentae of idiopathic small for gestational age infants Placenta 1982;3:309–317

    PubMed  CAS  Google Scholar 

  3. Salafia CM, et al. Placental pathology at term associated with elevated midtrimester maternal serum alpha-fetoprotein concentration Am J Obstet Gynecol 1988;158:1064–1066

    PubMed  CAS  Google Scholar 

  4. Redline RW, Abramowsky CR. Clinical and pathologic aspects of recurrent placental villitis Hum Pathol 1985;16:727–731

    PubMed  CAS  Google Scholar 

  5. Althabe O, Labarrere C. Chronic villitis of unknown aetiology and intrauterine growth-retarded infants of normal and low ponderal index Placenta 1985;6:369–373

    PubMed  CAS  Google Scholar 

  6. Redline RW, O’Riordan MA. Placental lesions associated with cerebral palsy and neurologic impairment following term birth Arch Pathol Lab Med 2000;124:1785–1791

    PubMed  CAS  Google Scholar 

  7. Jacques SM, Qureshi F. Chronic chorioamnionitis: a clinicopathologic and immunohistochemical study Hum Pathol 1998;29:1457–1461

    Article  PubMed  CAS  Google Scholar 

  8. Russell P, et al. Recurrent reproductive failure due to severe placental villitis of unknown etiology J Reprod Med 1980;24:93–98

    PubMed  CAS  Google Scholar 

  9. Benirschke K, et al. Villitis of known origin: varicella and toxoplasma Placenta 1999;20:395–399

    Article  PubMed  CAS  Google Scholar 

  10. Popek EJ. Granulomatous villitis due to Toxoplasma gondii Pediatr Pathol 1992;12:281–288

    PubMed  CAS  Google Scholar 

  11. Qureshi F, et al. Placental histopathology in syphilis Hum Pathol 1993;24:779–784

    Article  PubMed  CAS  Google Scholar 

  12. Qureshi F, Jacques SM. Maternal varicella during pregnancy: correlation of maternal history and fetal outcome with placental histopathology Hum Pathol 1996;27:191–195

    Article  PubMed  CAS  Google Scholar 

  13. Nakamura Y, et al. Detection of the human cytomegalovirus gene in placental chronic villitis by polymerase chain reaction Hum Pathol 1994;25:815–818

    Article  PubMed  CAS  Google Scholar 

  14. Labarrere CA, Althabe OH. Intrauterine growth retardation of unknown etiology: II. Serum complement and circulating immune complexes in maternal sera and their relationship with parity and chronic villitis Am J Reprod Immunol Microbiol 1986;12:4–6

    PubMed  CAS  Google Scholar 

  15. Labarrere C, et al. Deficiency of blocking factors in intrauterine growth retardation and its relationship with chronic villitis Am J Reprod Immunol Microbiol 1986;10:14–19

    PubMed  CAS  Google Scholar 

  16. Crum CP, et al. Chronic endometritis: the role of immunohistochemistry in the detection of plasma cells Am J Obstet Gynecol 1983;147:812–815

    PubMed  CAS  Google Scholar 

  17. Relman DA, et al. Identification of the uncultured bacillus of Whipple’s disease N Engl J Med 1992;327:293–301

    PubMed  CAS  Google Scholar 

  18. Relman DA, et al. Phylogenetic relationships among the agent of bacillary angiomatosis, Bartonella bacilliformis, and other alpha-proteobacteria Mol Microbiol 1992;6:1801–1807

    PubMed  Google Scholar 

  19. Knox CM, et al. 16S ribosomal DNA typing for identification of pathogens in patients with bacterial keratitis J Clin Microbiol 1998;36:3492–3496

    PubMed  CAS  Google Scholar 

  20. Knox CM, et al. Identification of bacterial pathogens in patients with endophthalmitis by 16S ribosomal DNA typing Am J Ophthalmol 1999;128:511–512

    Article  PubMed  CAS  Google Scholar 

  21. Wilck MB, et al. Endocarditis caused by culture-negative organisms visible by Brown and Brenn staining: utility of PCR and DNA sequencing for diagnosis J Clin Microbiol 2001;39:2025–2027

    Article  PubMed  CAS  Google Scholar 

  22. Redline RW. Recurrent villitis of bacterial etiology Pediatr Pathol Lab Med 1996;16:995–1001

    PubMed  CAS  Google Scholar 

  23. Stern RA, et al. Analysis of chronic endometritis for Chlamydia trachomatis by polymerase chain reaction Hum Pathol 1996;27:1085–1088

    Article  PubMed  CAS  Google Scholar 

  24. Rank RG, et al. Characterization of chlamydial genital infection resulting from sexual transmission from male to female guinea pigs and determination of infectious dose Infect Immun 2003;71:6148–6154

    Article  PubMed  CAS  Google Scholar 

  25. Saetta A, et al. Determination of CMV placentitis. Diagnostic application of the polymerase chain reaction Virchows Arch 1998;432:159–162

    Article  PubMed  CAS  Google Scholar 

  26. Kumazaki K, et al. Detection of cytomegalovirus DNA in human placenta J Med Virol 2002;68:363–369

    Article  PubMed  CAS  Google Scholar 

  27. Ozono K, et al. Diagnosis of congenital cytomegalovirus infection by examination of placenta: application of polymerase chain reaction and in situ hybridization Pediatr Pathol Lab Med 1997;17:249–258

    Article  PubMed  CAS  Google Scholar 

  28. Dong ZW, et al. Detection of congenital cytomegalovirus infection by using chorionic villi of the early pregnancy and polymerase chain reaction Int J Gynaecol Obstet 1994;44:229–231

    Article  PubMed  CAS  Google Scholar 

  29. Nyman M, et al. Detection of human parvovirus B19 infection in first-trimester fetal loss Obstet Gynecol 2002;99(5 pt 1):795–798

    PubMed  Google Scholar 

  30. Dong ZW, et al. Detection of a human parvovirus intrauterine infection with the polymerase chain reaction J Reprod Med 2000;45:410–412

    PubMed  CAS  Google Scholar 

  31. Eppel W, et al. Human papillomavirus in the cervix and placenta Obstet Gynecol 2000;96:337–341

    Article  PubMed  CAS  Google Scholar 

  32. Dong ZW, et al. Detection of Chlamydia trachomatis intrauterine infection using polymerase chain reaction on chorionic villi Int J Gynaecol Obstet 1998;61:29–32

    Article  PubMed  CAS  Google Scholar 

  33. Gencay M, et al. Chlamydia trachomatis detected in human placenta J Clin Pathol 1997;50:852–855

    PubMed  CAS  Google Scholar 

  34. Genen L, et al. Correlation of in situ detection of infectious agents in the placenta with neonatal outcome J Pediatr 2004;144:316–320

    PubMed  Google Scholar 

  35. Satosar A, et al. Histologic correlates of viral and bacterial infection of the placenta associated with severe morbidity and mortality in the newborn Hum Pathol 2004;35:536–545

    Article  PubMed  Google Scholar 

  36. Kraus FT, et al. Placental pathology Washington, DC: American Registry of Pathology, 2004;102

    Google Scholar 

  37. Schoeb TR, et al. Detection of urogenital mycoplasmal infections in primates by use of polymerase chain reaction Lab Anim Sci 1997;47:468–471

    PubMed  CAS  Google Scholar 

  38. Abele-Horn M, et al. Polymerase chain reaction versus culture for detection of Ureaplasma urealyticum and Mycoplasma hominis in the urogenital tract of adults and the respiratory tract of newborns Eur J Clin Microbiol Infect Dis 1996;15:595–598

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Linda M. Ernst.

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This work was presented at the interim meeting of the Society for Pediatric Pathology; Cincinnati, Ohio; October 2003.

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Ernst, L.M., Crouch, J., Rinder, H. et al. Bacterial Etiology for Chronic Villitis Is Not Supported by Polymerase Chain Reaction for 16S rRNA DNA. Pediatr Dev Pathol 8, 647–653 (2005). https://doi.org/10.1007/s10024-005-0412-1

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  • DOI: https://doi.org/10.1007/s10024-005-0412-1

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