Soils are a major component of the global C cycle, and considerable effort has been dedicated to improve our understanding of factors controlling soil organic C (SOC) turnover and stabilization in the last decades. Carbon stable isotopes are useful in this respect as they represent an integrative indicator of SOC biogeochemical processing. In the present study, C concentration and δ13C were measured in soil horizons of 21 forest sites located at the transition zone between the hardwood and the conifer forest in Québec, Canada, and related to 13 biophysical variables to identify the main drivers of SOC storage and turnover. Carbon concentrations in the forest floor (FF) and the B- and C-horizons were, respectively, strongly correlated with percentage of clay (Pclay), the mean annual precipitation: potential evapotranspiration ratio (MAP:PET), and percentage of hardwoods (Phwd). In FF, δ13C was poorly correlated with the studied variables, whereas in mineral horizons, it was significantly correlated with mean annual air temperature (MAAT) and the percentage of conifers (Pc) and Pclay. Across the studied area, δ13C increased on average by 2.0‰ from the FF to the C-horizon. The isotopic enrichment with soil depth (β) was strongly negatively correlated with Pc, which explained 55% of its variability among sites. This suggests that the vegetation type is an important driver of soil C long-term turnover rate in forest ecosystems. Overall, our data suggest that hardwood forest expansion in response to climate change might reduce the stability and the storage of SOC in the future.
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Aerts R, Chapin FI. 2000. The mineral nutrition of wild plants revisited: a re-evaluation of processes and patterns. Adv Ecol Res 30:1–67.
Arp PA, Yin XW. 1992. Predicting water fluxes through forests from monthly precipitation and mean monthly air temperature records. Can J For Sci 22:864–77.
Billings SA, Richter DD. 2006. Changes in stable isotopic signatures of soil nitrogen and carbon during 40 years of forest development. Oecologia 148:325–33.
Borchers J, Perry D. 1992. The influence of soil texture and aggregation on carbon and nitrogen dynamics in southwest Oregon forests and clear cuts. Can J For Res 22:298–305.
Borken W, Ahrens B, Schulz C, Zimmermann L. 2011. Site-to-site variability and temporal trends of DOC concentrations and fluxes in temperate forest soils. Glob Chang Biol 17:2428–43.
Boström B, Comstedt D, Ekblad A. 2007. Isotope fractionation and 13C enrichment in soil profiles during the decomposition of soil organic matter. Oecologia 153:89–98.
Chapin FS, Peterson G, Berkes F, Callaghan TV, Angelstam P, Apps M, Beier C, Bergeron Y, Crépin A-S, Danell K, Elmqvist T, Folke C, Forbes B, Fresco N, Juday G, Niemelä J, Shvidenko A, Whiteman G. 2004. Resilience and vulnerability of northern regions to social and environmental change. Ambio 33:344–9.
Cramer W, Bondeau A, Woodward FI, Prentice IC, Betts RA, Brovkin V, Cox PM, Fisher V, Foley JA, Friend AD, Kucharik C, Lomas MR, Ramankutty N, Sitch S, Smith B, White A, Young-Molling C. 2001. Global response of terrestrial ecosystem structure and function to CO2 and climate change: results from six dynamic global vegetation models. Glob Chang Biol 7:357–73.
Davidson EA, Janssens IA. 2006. Temperature sensitivity of soil carbon decomposition and feedbacks to climate change. Nature 440:165–73.
De Deyn GB, Cornelissen JHC, Bardgett RD. 2008. Plant functional traits and soil carbon sequestration in contrasting biomes. Ecol Lett 11:516–31.
Dijkstra P, Ishizu A, Doucett R, Hart SC, Schwartz E, Menyailo OV, Hungate BA. 2006. 13C and 15N natural abundance of the soil microbial biomass. Soil Biol Biochem 38:3257–66.
Diochon A, Kellman L, Beltrami H. 2009. Looking deeper: an investigation of soil carbon losses following harvesting from a managed northeastern red spruce (Picea rubens Sarg.) forest chronosequence. For Ecol Manage 257:413–20.
Dorodnikov M, Fangmeier A, Kuzyakov Y. 2007. Effects of atmospheric CO2 enrichment on δ13C, δ15N values and turnover times of soil organic matter pools isolated by thermal techniques. Plant Soil 297:15–28.
Doucet A, Savard MM, Bégin C, Marion J, Smirnoff A, Ouarda T. 2012. Combining tree-ring metal concentrations and lead, carbon and oxygen isotopes to reconstruct peri-urban atmospheric pollution. Tellus Ser B-Chem Phys Meteorol 64:1–18.
Dungait JAJ, Hopkins DW, Gregory AS, Whitmore AP. 2012. Soil organic matter turnover is governed by accessibility not recalcitrance. Glob Chang Biol 18:1781–96.
Ehleringer JR, Buchmann N, Flanagan LB. 2000. Carbon isotope ratios in below ground carbon cycle processes. Ecol Appl 10:412–22.
Farquhar GD, Ehleringer JR, Hubick KT. 1989. Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–37.
Follett RF, Stewart CE, Pruessner EG, Kimble JM. 2012. Effects of climate change on soil carbon and nitrogen storage in the US Great Plains. J Soil Water Conserv 67:331–42.
Fontaine S, Barot S, Barré P, Bdioui N, Mary B, Rumpel C. 2007. Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature 450:277–80.
Francey RJ, Allison CE, Etheridge DM, Trudinger CM, Enting IG, Leuenberger M, Langenfelds RL, Michel E, Steele LP. 1999. A 1000-year high precision record of delta C-13 in atmospheric CO2. Tellus Ser B-Chem Phys Meteorol 51:170–93.
Friedli H, Lotscher H, Oeschger H, Siegenthaler U, Stauffer B. 1986. Ice core record of the 13C/12C ratio of atmospheric CO2 in the past two centuries. Nature 324:237–8.
Fröberg M, Hansson K, Kleja DB, Alavi G. 2011. Tree species effects on soils in temperate and boreal forests: emerging themes and research needs. For Ecol Manage 262:1742–7.
Garten CT, Cooper LW, Post WM, Hanson PJ. 2000. Climate controls on forest soil C isotope ratios in the Southern Appalachian Mountains. Ecology 81:1108–19.
Garten CT, Hanson PJ. 2006. Measured forest soil C stocks and estimated turnover times along an elevation gradient. Geoderma 136:342–52.
Garten CT, Post W, Hanson PJ, Cooper L. 1999. Forest soil carbon inventories and dynamics along an elevation gradient in the southern Appalachian Mountains. Biogeochemistry 45:115–45.
Garten CT. 2006. Relationships among forest soil C isotopic composition, partitioning, and turnover times. Can J For Res 36:2157–67.
Garten CT. 2011. Comparison of forest soil carbon dynamics at five sites along a latitudinal gradient. Geoderma 167–168:30–40.
Gebauer G, Schulze E-D. 1991. Carbon and nitrogen isotope ratios in different compartments of a healthy and a declining Picea abies forest in the Fichtelgebirge, NE Bavaria. Oecologia 87:198–207.
Gotelli NJ, Ellison AM. 2002. Biogeography at a regional scale: determinants of ant species density in New England bogs and forests. Ecology 83:1604–9.
Hélie JF. 2009. Elemental and stable isotopic approaches for studying the organic and inorganic carbon components in natural samples. From Deep-Sea to Costal Zones: Methods-Techniques for Studying Paleoenvironments. IOP Conf Ceries Earth. Environ Sci 5:012205.
Högberg P, Ekblad A, Nordgren A, Plamboeck A, Ohlsson A, Bhupinderpal-Singh HM. 2005. Factors determining the 13C abundance of soil-respired CO2 in boreal forests. In: Flanagan L, Ehleringer J, Pataki D, Eds. Stable Isotopes and Biosphere Atmosphere Interactions. New York: Elsevier. p 47–68.
Högberg P, Plamboeck AH, Taylor AF, Fransson PM. 1999. Natural (13)C abundance reveals trophic status of fungi and host-origin of carbon in mycorrhizal fungi in mixed forests. Proc Natl Acad Sci USA 96:8534–9.
Houghton RA. 2007. Balancing the global carbon budget. Annu Rev Earth Planet Sci 35:313–47.
Houle D, Bouffard A, Duchesne L, Logan T, Harvey R. 2012a. Projections of future soil temperature and water content for three Southern Quebec forested sites. J Clim 25:7690–701.
Houle D, Duchesne L, Ouimet R, Paquin R, Meng FR, Arp PA. 2002. Evaluation of the FORHYM2 model for prediction of hydrologic fluxes and soil temperature at the Lake Clair Watershed (Duchesnay, Quebec). For Ecol Manage 159:249–60.
Houle D, Lamoureux P, Bélanger N, Bouchard M, Gagnon C, Couture S, Bouffard A. 2012b. Soil weathering rates in 21 catchments of the Canadian Shield. Hydrol Earth Syst Sci 16:685–97.
Houle D, Ouimet R, Couture S, Gagnon C. 2006. Base cation reservoirs in soil control the buffering capacity of lakes in forested catchments. Can J Fish Aquat Res 63:471–4.
Iverson LR, Prasad AM, Matthews SN, Peters M. 2008. Estimating potential habitat for 134 eastern US tree species under six climate scenarios. For Ecol Manage 254:390–406.
Jobbagy EG, Jackson RB. 2000. The vertical distribution of soil organic carbon and its relation to climate and vegetation. Ecol Appl 10:423–36.
Kleber M, Nico PS, Plante A, Filley T, Kramer M, Swanston C, Sollins P. 2011. Old and stable soil organic matter is not necessarily chemically recalcitrant: implications for modeling concepts and temperature sensitivity. Glob Chang Biol 17:1097–107.
Kramer MG, Sollins P, Sletten RS, Swart PK. 2003. N isotope fractionation and measures of organic matter alteration during decomposition. Ecology 84:2021–5.
Lachance M, Bobée B, Grimard Y. 1985. Sensitivity of southern Québec lakes to acidic precipitation. Water Air Soil Pollut 25:115–32.
Laganière J, Paré D, Bergeron Y, Chen HYH, Brassard BW, Cavard X. 2013. Stability of soil carbon stocks varies with forest composition in the Canadian boreal biome. Ecosystems 16:852–65.
Laganière J, Paré D, Bradley RL. 2010. How does a tree species influence litter decomposition? Separating the relative contribution of litter quality, litter mixing, and forest floor conditions. Can J For Res 40:465–75.
Lal R. 2005. Forest soils and carbon sequestration. For Ecol Manage 220:242–58.
Leonardi S, Gentilesca T, Guerrieri R, Ripullone F, Magnani F, Mencuccini M, Noije TV, Borghetti M. 2012. Assessing the effects of nitrogen deposition and climate on carbon isotope discrimination and intrinsic water-use efficiency of angiosperm and conifer trees under rising CO2 conditions. Glob Chang Biol 18:2925–44.
Mariotti A, Germon JC, Hubert P, Kaiser P, Letolle R, Tardieux A, Tardieux P. 1981. Experimental determination of nitrogen kinetic isotope fractionation: some principles; illustration for the denitrification and nitrification processes. Plant Soil 62:413–30.
Marty C, Houle D, Gagnon C, Duchesne L. 2011. Isotopic compositions of S, N and C in soils and vegetation of three forest types in Quebec, Canada. Appl Geochem 26:2181–90.
McKeague JA, Ed. 1978. Manual on soil sampling and methods of analysis. 3rd edn. Ottawa, Ont: Soil Research Institute, Agriculture Canada. 212 pp
Michalzik B, Kalbitz K, Park J, Solinger S. 2001. Fluxes and concentrations of dissolved organic carbon and nitrogen—a synthesis for temperate forests. Biogeochemistry 52:173–205.
Nadelhoffer KJ, Fry B. 1988. Controls on natural nitrogen-15 and carbon-13 abundances in forest soil organic matter. Madison, WI, USA: Soil Science Society of America.
Natelhoffer KJ, Fry B. 1988. Controls on natural Nitrogen-15 and Carbon-13 abundances in forest soil organic matter. 52:1633–40.
Neff JC, Townsend AR, Gleixner G, Lehman SJ, Turnbull J, Bowman WD. 2002. Variable effects of nitrogen additions on the stability and turnover of soil carbon. Nature 419:915–17.
Notaro M, Vavrus S, Liu Z. 2007. Global vegetation and climate change due to future increases in CO2 as projected by a fully coupled model with dynamic vegetation. J Clim 20:70–90.
Novák M, Buzek F, Harrison AF, Prechova E, Jacková I, Fottová D. 2003. Similarity between C, N and S stable isotope profiles in European spruce forest soils: implications for the use of delta 34S as a tracer. Appl Geochem 18:765–79.
Périé C, Ouimet R. 2008. Organic carbon, organic matter and bulk density relationships in boreal forest soils. Can J Soil Sci 88:315–25.
Powers J, Schlesinger W. 2002. Relationships among soil carbon distributions and biophysical factors at nested spatial scales in rainforests of northeastern Costa Rica. Geoderma 109:165–90.
Quideau S, Chadwick O, Benesi A, Graham R, Anderson M. 2001a. A direct link between forest vegetation type and soil organic matter composition. Geoderma 104:41–60.
Quideau SA, Chadwick OA, Trumbore SE, Johnson-Maynard JL, Graham RC, Anderson MA. 2001b. Vegetation control on soil organic matter dynamics. Org Geochem 32:247–52.
Régnière J, Bolstad P. 1994. Statistical simulation of daily air temperature patterns eastern North America to forecast seasonal events in insect pest management. Environ Entomol 23:1368–80.
Régnière J, St-Amant R. 2007. Stochastic simulation of daily air temperature and precipitation from monthly normals in North America north of Mexico. Int J Biometeorol 51:415–30.
Régnière J. 1996. Generalized approach to landscape-wide seasonal forecasting with temperature-driven simulation models. Environ Entomol 25:869–81.
Renée J, Lawrence B, Buchmann N, Ehleringer JR, Brooks J, Flanagan L. 1997. Carbon isotope composition of boreal plants: functional grouping of life forms. Oecologia 110:301–11.
Rumpel C, Kögel-Knabner I. 2011. Deep soil organic matter—a key but poorly understood component of terrestrial C cycle. Plant Soil 338:143–58.
Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögel-Knabner I, Lehmann J, Manning DAC, Nannipieri P, Rasse DP, Weiner S, Trumbore SE. 2011. Persistence of soil organic matter as an ecosystem property. Nature 478:49–56.
Tremblay S, Ouimet R, Houle D. 2002. Prediction of organic carbon content in upland forest soils of Quebec, Canada. Can J For Res 914:903–4.
Trumbore S, Harden J. 1997. Accumulation and turnover of carbon in organic and mineral soils of the BOREAS northern study area. J Geophys Res 102:817–30.
Trumbore S. 1997. Potential responses of soil organic carbon to global environmental change. Proc Natl Acad Sci USA 94:8284–91.
Vergutz L, Manzoni S, Porporato A, Novais R, Jackson R. 2012. Carbon isotope discrimination and photosynthesis. Ecol Monogr 82:205–20.
Vesterdal L, Elberling B, Christiansen JR, Callesen I, Schmidt IK. 2012. Soil respiration and rates of soil carbon turnover differ among six common European tree species. For Ecol Manage 264:185–96.
Wang N, Xu SS, Jia X, Gao J, Zhang WP, Qiu YP, Wang GX. 2013. Variations in foliar stable carbon isotopes among functional groups and along environmental gradients in China—a meta-analysis. Plant Biol 15:144–51.
Yin XW, Arp PA. 1993. Predicting forest soil temperatures from monthly air temperature and precipitation records. Can J For Sci 23:2521–36.
Yurova A, Lankreijer H. 2007. Carbon storage in the organic layers of boreal forest soils under various moisture conditions: a model study for Northern Sweden sites. Ecol Modell 204:475–84.
This research was supported by Environment Canada’s Acid Rain program, CARA, and the Ministère des Forêts, de la Faune et des Parcs du Québec (MFFP). We are also thankful to the MFFP laboratory for soil analysis.
CM contributed in study design, analyzed data, and wrote the paper. DH conceived of the study and contributed in data analysis and in writing the paper. CG contributed to research and manuscript development.
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Marty, C., Houle, D. & Gagnon, C. Effect of the Relative Abundance of Conifers Versus Hardwoods on Soil δ13C Enrichment with Soil Depth in Eastern Canadian forests. Ecosystems 18, 629–642 (2015). https://doi.org/10.1007/s10021-015-9852-2
- forest soils
- soil organic carbon
- carbon turnover time
- isotopic enrichment factor
- boreal forests