Abstract
Nutrient nitrogen and water availability are co-limiting factors for grassland productivity in the Inner Mongolian steppe. The objective of this study was to evaluate the interactive effects of water and nitrogen (N) addition on soil abiotic factors and soil nematode community composition. A 3-year experiment with addition of water (with and without irrigation simulating wet year precipitation) and nitrogen (0, 25, and 50 kg N ha−1) was conducted at two sites in Inner Mongolia with histories of heavy grazing (HG) and moderate grazing (MG). The results showed that HG had several lower nematode ecological indicators; namely, the Simpson index (P < 0.01), maturity index (MI, including free-living nematodes), and plant parasite index (PPI, including plant-parasitic nematodes). In addition, HG had a lower proportion of omnivores–predators and higher proportion of bacterivores (P < 0.01), with reduced soil moisture, total nitrogen and total carbon (C), and increased C/N ratio. A relatively low addition of N did not influence soil total carbon and total nitrogen and had only a slight effect on the composition and structure of the soil nematode community. Water addition increased the proportion of plant parasites, reduced the proportion of bacterivores and omnivores–predators and increased the soil total carbon and total nitrogen. Redundancy analysis revealed that grazing history alone explained more variation in nematode taxon composition (17.4%, P < 0.01) and PPI (35.6%, P < 0.01) and MI (17.5%, P < 0.01) and Simpson index (10.5%, P < 0.01). Water addition explained more variation in the nematode feeding group (24.4%, P < 0.01), total nematodes (7.9%, P < 0.01) and PPI (78.7%, P < 0.01). Nitrogen addition explained variation in the nematode taxon composition (6.2%, P < 0.01). These results suggested that HG caused soil degradation, and water addition facilitated plant parasites and enhanced soil properties, whereas application of small amounts of nitrogen to N-limited semiarid grasslands did not induce clear changes in this system in the short term.
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References
Bai YF, Han XG, Wu JG, Chen ZZ, Li LH. 2004. Ecosystem stability and compensatory effects in the Inner Mongolia grassland. Nature 431:181–4.
Bai YF, Wu JG, Xing Q, Pan QM, Huang JH, Yang DL, Han XG. 2008. Primary production and rain use efficiency across a precipitation gradient on the Mongolia plateau. Ecology 89:2140–53.
Bai YF, Wu JG, Clark CM, Naeem S, Pan QM, Huang JH, Zhang LX, Han XG. 2010. Tradeoffs and thresholds in the effects of nitrogen addition on biodiversity and ecosystem functioning: evidence from Inner Mongolia Grasslands. Global Change Biol 16:358–72.
Bao SD. 2000. Soil and agricultural chemistry analysis. Beijing: China Agricultural Press (in Chinese).
Bardgett RD, Jones AC, Jones DL, Kemmitt SJ, Cook R, Hobbs PJ. 2001. Soil microbial community patterns related to the history and intensity of grazing in sub-montane ecosystems. Soil Biol Biochem 33:1653–64.
Bongers T. 1990. The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83:14–19.
Chen Q. 2011. The response of net primary production and plant species composition to the interaction of water and nitrogen in typical steppe, Inner Mongolia. Dissertation. pp 40–66.
Chen Q, Hooper DU, Lin S. 2011. Shifts in species composition constrain restoration of overgrazed grassland using nitrogen fertilization in Inner Mongolian steppe. China. Plos One 6(3):e16909.
Cheng Z, Grewal PS, Stinner BR, Hurto KA, Hamza HB. 2008. Effects of long-term turfgrass management practices on soil nematode community and nutrient pools. Appl Soil Ecol 38:174–84.
Clark CM, Tilman D. 2008. Loss of plant species after chronic low-level nitrogen deposition to prairie grasslands. Nature 451:712–15.
De Deyn GB, Raaijmakers CE, Zoomer HR, Berg MP, de Ruiter PC, Verhoef HA, Bezemer TM, van der Putten WH. 2003. Soil invertebrate fauna enhances grassland succession and diversity. Nature 422:711–13.
De Deyn GB, Raaijmakers CE, van Ruijven J, Berendse F, van der Putten WH. 2004. Plant species identity and diversity effects on different trophic levels of nematodes in the soil food web. Oikos 106:576–86.
De Ruiter PC, Moore JC, Zwart KB, Bouwman LA, Hassink J, Bloem J, De Vos JA, Marinissen JCY, Didden WAM, Lebbink G, Brussaard L. 1993. Simulation of nitrogen mineralization in the below-ground food webs of two winter wheat fields. J Appl Ecol 30:95–106.
Ferris H. 2010. Form and function: metabolic footprints of nematodes in the soil food web. Eur J Soil Biol 46:97–104.
Ferris H, Matute MM. 2003. Structural and functional succession in the nematode fauna of a soil food web. Appl Soil Ecol 23:93–110.
Ferris H, Venette RC, van der Meulen HR, Lau SS. 1998. Nitrogen mineralization by bacterial-feeding nematodes: verification and measurement. Plant Soil 203:159–71.
Ferris H, Venette RC, Scow KM. 2004. Soil management to enhance bacterivore and fungivore nematode populations and their nitrogen mineralisation function. Appl Soil Ecol 25:19–35.
Freckman DW, Ettema CH. 1993. Assessing nematode communities in agroecosystems of varying human intervention. Agric Ecosyst Environ 45:239–61.
Freckman DW, Whitford WG, Steinberger Y. 1987. Effect of irrigation on nematode population dynamics and activity in desert soils. Biol Fert Soils 3:3–10.
Gong XY, Chen Q, Lin S, Brueck H, Dittert K, Taube F, Schnyder H. 2011. Tradeoffs between nitrogen- and water-use efficiency in dominant species of the semiarid steppe of Inner Mongolia. Plant Soil 340:227–38.
Kardol P, Cregger MA, Campany CE, Classen AT. 2010. Soil ecosystem functioning under climate change: plant species and community effects. Ecology 91:767–81.
Khan Z, Kim YH. 2005. The predatory nematode, Mononchoides fortidens (Nematoda: Diplogasterida), suppresses the root-knot nematode, Meloidogyne arenaria, in potted field soil. Biol Control 35:78–82.
Landesman WJ, Treonis AM, Dighton J. 2011. Effects of a one-year rainfall manipulation on soil nematode abundances and community composition. Pedobiologia 54:87–91.
Li JZ, Lin S, Taube F, Pan QM, Dittert K. 2011. Above and belowground net primary productivity of grassland influenced by supplemental water and nitrogen in Inner Mongolia. Plant Soil 340:253–64.
Liang WJ, Lou YL, Li Q, Zhong S, Zhang XK, Wang JK. 2009. Nematode faunal response to long-term application of nitrogen fertilizer and organic manure in Northeast China. Soil Biol Biochem 41:883–90.
Mills AAS, Adl MS. 2011. Changes in nematode abundances and body length in response to management intensive grazing in a low-input temperate pasture. Soil Biol Biochem 43:150–8.
Nan ZB. 2005. The grassland farming system and sustainable agricultural development in China. Grassland Sci 51:15–19.
Niu SL, Yang HJ, Zhang Z, Wu MY, Lu Q, Li LH, Han XG, Wan SQ. 2009. Non-additive effects of water and nitrogen addition on ecosystem carbon exchange in a temperate steppe. Ecosystems 12:915–26.
Olff H, Hoorens B, de Goede RGM, van der Putten WH, Gleichman JM. 2000. Small-scale shifting mosaics of two dominant grassland species: the possible role of soil-borne pathogens. Oecologia 125:45–54.
Pan QM, Bai YF, Wu JG, Han XG. 2011. Hierarchical plant responses and diversity loss after nitrogen addition: testing three functionally-based hypotheses in the Inner Mongolia grassland. PLoS ONE 6(5):e20078.
Paul KI, Polglase PJ, O’Connell AM, Carlyle JC, Smethurst PJ, Khanna PK. 2003. Defining the relation between soil water content and net nitrogen mineralization. Eur J Soil Sci 54:39–47.
Pineiro G, Paruelo JM, Oesterheld M, Jobbagy EG. 2010. Pathways of grazing effects on soil organic carbon and nitrogen. Rangel Ecol Manag 63:109–19.
Porazinska DL, et al., 2003. Relationships at the aboveground-belowground interface: plants, soil biota, and soil processes. Ecol Monogr 73:377–395.
Qi S, Zheng HX, Lin QM, Li GT, Xi ZH, Zhao XR. 2011. Effects of livestock grazing intensity on soil biota in a semiarid steppe of Inner Mongolia. Plant Soil 340:117–26.
Sánchez-Moreno S, Minoshima H, Ferris H, Jackson LE. 2006. Linking soil properties and nematode community composition: effects of soil management on soil food webs. Nematology 8:703–15.
Steffens M, KÖlbl A, Totsche KU, KÖgel-Knabner I. 2008. Grazing effects on soil chemical and physical properties in a semiarid steppe of Inner Mongolia (P.R. China). Geoderma 143:63–72.
ter Braak CJF, Šmilauer P. 2002. Canoco for Windows 4.5. Wageningen: Biometris.
Veen GF, Olff H, Duyts H, van der Putten WH. 2010. Vertebrate herbivores influence soil nematodes by modifying plant communities. Ecology 91:828–35.
Viketoft M, Bengtsson J, Sohlenius B, Berg MP, Petchey O, Palmborg C, Huss-Danell K. 2009. Long-term effects of plant diversity and composition on soil nematode communities in model grasslands. Ecology 90:90–9.
Wang CH, Wan SQ, Xing XR, Zhang L, Han XG. 2006. Temperature and soil moisture interactively affected soil net N mineralization in temperate grassland in Northern China. Soil Biol Biochem 38:1101–10.
Wardle DA, Bardgett RD, Klironomos JN, Setala H, van der Putten WH, Wall DH. 2004. Ecological linkages between aboveground and belowground biota. Science 304:1629–33.
Xia JY, Niu SL, Wan SQ. 2009. Response of ecosystem carbon exchange to warming and nitrogen addition during two hydrologically contrasting growing seasons in a temperate steppe. Global Change Biol 15:1544–56.
Yeates GW, Bongers T. 1999. Nematode diversity in agroecosystems. Agric Ecosyst Environ 74:113–35.
Yeates GW, Bongers T, de Goede RGM, Freckman DW, Georgieva SS. 1993. Feeding habits in soil nematode families and genera: an outline for soil ecologists. J Nematol 25:315–31.
Zhao Y, Peth S, Reszkowska A, Gan L, Krummelbein J, Peng XH, Horn R. 2011. Response of soil moisture and temperature to grazing intensity in a Leymus chinensis steppe, Inner Mongolia. Plant Soil 340:89–102.
Acknowledgements
We thank the National Basic Research Program of China (973 Program) (2007CB106802) and the National Nature Science Foundation of China (41071207). We also thank the Inner Mongolia Grassland Ecosystem Research Station of Botany Institute, Chinese Academy of Science, for providing working facilities. We gratefully acknowledge Professor Wim H. van der Putten for valuable comments on the manuscript.
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Wei-bin Ruan and Yuan Sang contributted equally to this work.
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Wei-bin Ruan wrote the paper and performed the research. Yuan Sang: performed the research. Qin Chen: performed the research. Xiang Zhu analyzed data. Shan Lin conceived of or designed study. Yu-bao Gao conceived of or designed study.
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Ruan, Wb., Sang, Y., Chen, Q. et al. The Response of Soil Nematode Community to Nitrogen, Water, and Grazing History in the Inner Mongolian Steppe, China. Ecosystems 15, 1121–1133 (2012). https://doi.org/10.1007/s10021-012-9570-y
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DOI: https://doi.org/10.1007/s10021-012-9570-y