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The Elevated Level of Circulating Matrix Metalloproteinase-9 in Patients with Abdominal Aortic Aneurysms Decreased to Levels Equal to Those of Healthy Controls after an Aortic Repair

  • Clinical Research
  • Published:
Annals of Vascular Surgery

Abstract

Matrix metalloproteinase-9 (MMP-9) is abundantly expressed in abdominal aortic aneurysms (AAAs), and it is considered to play a pivotal role in aneurysmal formation. Elevated circulating concentrations of MMP-9 have been reported in patients with AAA, but the influence of an operation on circulating MMP-9 is unclear. Therefore, to clarify the influence of an operation on circulating MMP-9 levels and to determine the role of MMP-9 in the progression of AAA, we measured serum MMP-9 levels in patients before and after AAA repair. Blood samples were obtained from 53 patients with AAA; 22 patients underwent AAA operations, including 17 patients with arterial occlusive disease (AOD), and nine normal control subjects. Serum MMP-9 levels were determined by an enzyme-linked immunosorbent assay. The serum MMP-9 concentration was significantly higher in AAA patients (622.0 ± 400.2 ng/mL) than in either AOD patients (284.3 ± 151.4 ng/mL) or healthy controls (280.8 ± 165.5 ng/mL) (p < 0.001). The mean serum MMP-9 levels in patients undergoing surgery for AAA (268.1 ± 215.9 ng/mL) was significantly lower than that in AAA patients (p < 0.01). Among the 10 patients whose sera were obtained preoperatively and postoperatively, the serum MMP-9 concentration fell significantly after the patients underwent the operation (p = 0.004). No significant difference was identified in serum MMP-9 concentrations among AOD patients, controls, and postoperative patients. These studies suggest that MMP-9 plays a pivotal role in aneurysm formation, and the circulating MMP-9 level is thus considered to reflect the biological behavior of the aneurysm.

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Acknowledgement

The authors thank Kanebo Corporation (Osaka, Japan) for their generous contribution of the reagents for the MMP-9 assay.

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Authors and Affiliations

  1. Division of Advanced Surgical Science and Technology, Graduate School of Medicine, Tohoku University, Sendai, Japan

    Tetsuo Watanabe MD, PhD, Akira Sato MD, PhD, Hitoshi Goto MD, PhD, Hiroshi Yamashita MD, PhD, Daijirou Akamatsu MD, PhD, Hiroko Sato MD, PhD, Takuya Shimizu MD, Noriyuki Miyama MD, Yoshiyuki Nakano MD & Susumu Satomi MD, PhD

  2. First Department of Pathology, School of Medicine, Iwate Medical University, Morioka, Japan

    Takashi Sawai MD, PhD & Miwa Uzuki MD, PhD

  3. Division of Advanded Surgical Science and Technology, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, 980-8574, Aoba-ku, Sendai, Japan

    Tetsuo Watanabe MD, PhD

Authors
  1. Tetsuo Watanabe MD, PhD
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  2. Akira Sato MD, PhD
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  3. Takashi Sawai MD, PhD
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  4. Miwa Uzuki MD, PhD
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  5. Hitoshi Goto MD, PhD
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  6. Hiroshi Yamashita MD, PhD
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  7. Daijirou Akamatsu MD, PhD
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  8. Hiroko Sato MD, PhD
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  9. Takuya Shimizu MD
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  12. Susumu Satomi MD, PhD
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Corresponding author

Correspondence to Tetsuo Watanabe MD, PhD.

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Watanabe, T., Sato, A., Sawai, T. et al. The Elevated Level of Circulating Matrix Metalloproteinase-9 in Patients with Abdominal Aortic Aneurysms Decreased to Levels Equal to Those of Healthy Controls after an Aortic Repair. Ann Vasc Surg 20, 317–321 (2006). https://doi.org/10.1007/s10016-006-9038-7

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  • DOI: https://doi.org/10.1007/s10016-006-9038-7

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