Skip to main content
SpringerLink
Log in

Influence of Indomethacin in the Rat Aneurysm Model

  • Published:
Annals of Vascular Surgery

Abstract

The objective of this study was to trigger the formation of rat abdominal aortic aneurysm by applying calcium chloride periarterially and then to detect the degree of prevention of aneurysm occurrence by oral introduction of indomethacin in some of the rats. Thirty-one rats were divided into three groups. The infrarenal aorta above the iliac bifurcation of rats was treated with sodium chloride in group 1 (control, n = 7), calcium chloride in group 2 (n = 12), and calcium chloride-indomethacin in group 3 (n = 12) periarterially. The rats of each group were randomly selected at the end of the first, second, and third weeks postoperatively; and vessel diameters of abdominal aortas were measured by digital photography using a micrometer. Aneurysmal development was not observed in any of the rats in the control group. None of the comparisons was statistically significant (p > 0.05). Aneurysmal development was observed in all of the rats in the calcium chloride group. Results from the first, second, and third weeks postoperatively were statistically significant (p < 0.05). A middle aneurysmal development was observed in all rats in the calcium chloride-indomethacin group. Only results from the second and third weeks postoperatively were statistically significant (p < 0.05). Measurements in groups 2 and 3 were statistically significant when compared to group 1 (p < 0.001). However, the mean increase in the indomethacin-treated group (group 3) was only 26.1%. The macroscopic appearance of the control group and an aneurysm induced by calcium-chloride application are shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1. Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.

Similar content being viewed by others

References

  1. Tilson MD. Histochemistry of aortic elastin in patients with nonspecific abdominal aortic aneurysmal disease. Arch Surg 1988;123:503.

    PubMed  CAS  Google Scholar 

  2. Rizzo RJ, McCarthy WJ, Dixit SN, et al. Collagen types and matrix protein content in human abdominal aortic aneurysms. J Vasc Surg 1989;10:365.

    Article  PubMed  CAS  Google Scholar 

  3. Holmes DR, Petrinec D, Wester W, Thompson RW, Reilly JM. Indomethacin prevents elastase-induced abdominal aortic aneurysms in the rat. J Surg Res 1996;63:305–309.

    Article  PubMed  CAS  Google Scholar 

  4. Brophy CM, Reilly JM, Walker Smith GJ, Tilson MD. The role of inflammation in nonspecific aneurysm disease. Ann Vasc Surg 1991;5:299.

    Article  Google Scholar 

  5. Reilly JM, Sicard GA, Lucore CL. Abnormal expression of plasminogen activators in aortic aneurysmal and occlusive disease. J Vasc Surg 1994;19:865.

    PubMed  CAS  Google Scholar 

  6. Vine N, Powell JT. Metalloproteinases in degenerative aortic disease. Clin Sci (Colch.) 1991;81:233.

    CAS  Google Scholar 

  7. Basalyga DM, Simionescu DT, Xiong W, Baxter BT, Starcher BC, Vyavahare NR. Elastin degradation and calcification in an abdominal aorta injury model. Role of matrix metalloproteinases. Circulation 2004;110:3480–3487.

    Article  PubMed  CAS  Google Scholar 

  8. Tambiah J, Franklin IJ, Trendell-Smşth N, Peston D, Powell JT. Provocation of experimental aortic inflammation and dilatation by inflammatory mediators and Chlamydia pneumoniae. Br J Surg 2001;88:935–940.

    Article  PubMed  CAS  Google Scholar 

  9. Cronenweet JL, Krupski WC, Rutherford RB. Abdominal aortic and iliac aneurysms. In Rutherford RB, ed. Vascular Surgery, 5th ed. Philadelphia: Saunders, 2000, pp 1246–1280.

  10. Yamaguchi T, Yokokawa M, Suzuki M, et al. Morphologic changes in the aorta during elastase infusion in the rat aneurysm model. J Surg Res 2001;95:161–166.

    Article  PubMed  CAS  Google Scholar 

  11. Anidjar S, Salzmann JL, Gentric D, Lagneau P, Camilleri JP, Michel JB. Elastase-induced experimental aneurysms in rats. Circulation 1990;82:973–981.

    PubMed  CAS  Google Scholar 

  12. Anidjar S, Michel JB. Pathogenesis of acquired abdominal aortic aneurysms. Presse Med 1991;20:859–865.

    PubMed  CAS  Google Scholar 

  13. Thompson RW, Holmes DR, Mertens RA, et al. Production and localization of 92-kilodalton gelatinase in abdominal aortic aneurysms: an elastolytic metalloproteinase expressed by aneurysm-infiltrating macrophages. J Clin Invest 1995;96:318–326.

    Article  PubMed  CAS  Google Scholar 

  14. Jones KG, Brull DJ, Brown LC, et al. Interleukin-6 and the prognosis of abdominal aortic aneurysms. Circulation 2001;103:2260–2265.

    PubMed  CAS  Google Scholar 

  15. Walton LJ, Franklin IJ, Bayston T. Inhibition of prostaglandin E2 synthesis in abdominal aortic aneurysms. Circulation 1999;100:48–54.

    PubMed  CAS  Google Scholar 

  16. Franklin IJ, Walton LJ, Greenhalgh RM, Powell JT. The influence of indomethacin on the metabolism and cytokine secretion of human aneurysmal aorta. Eur J Vasc Endovasc Surg 1999;1:35–42.

    Article  Google Scholar 

  17. McMillan WD, Patterson BK, Keen RR, Shively BP, Cipollone M, Pearce WH. In situ localization and quantification of mRNA for 92-kD type IV collagenase and its inhibitor in aneurysmal, occlusive, and normal aorta. Arterioscler Thromb 1995;15:1139–1144.

    CAS  Google Scholar 

  18. Chiou AC, Chiu C, Pearce WH. Murine aortic aneurysm produced by periarterial application of calcium chloride. J Surg Res 2001;99:371–376.

    Article  PubMed  CAS  Google Scholar 

  19. Hall DA. Elastic tissue alterations in vascular disease. In: Blumenthal HT, ed. Cowdry’s Arteriosclerosis. Springfield, IL: Thomas, 1967, pp 121–140.

    Google Scholar 

  20. Yu SY. Elastic tissue and arterial calcification. In: Blumenthal HT ed. Cowdry’s Arteriosclerosis. Springfield, IL: Thomas, 1967, pp 170–192.

    Google Scholar 

  21. Gertz SD, Kurgan A, Eisenberg D. Aneurysm of the rabbit common carotid artery induced by periarterial application of calcium chloride in vivo. J Clin Invest 1988;81:649–656.

    Article  PubMed  CAS  Google Scholar 

  22. Anidjar S, Dobrin PB, Eichorst M, Graham GP, Chejfec G. Correlation of inflammatory infiltrate with the enlargement of experimental aortic aneurysms. J Vasc Surg 1992;16:139–147.

    Article  PubMed  CAS  Google Scholar 

  23. Halpern VJ, Nackman GB, Gandhi RH, et al. The elastase infusion model of experimental aortic aneurysm: synchrony of induction of endogeneous proteinases with matrix destruction and inflammatory cell response. J Vasc Surg 1994;20:51.

    PubMed  CAS  Google Scholar 

  24. Shah PK. Inflammation, metalloproteinases and increased proteolysis: an emerging pathophysiological paradigm in aortic aneurysm. Circulation 1997;96:2115–2117.

    PubMed  CAS  Google Scholar 

  25. Grange JJ, Davis V, Baxter BT. Pathogenesis of abdominal aortic aneurysm: an update and look toward the future. Cardiovasc Surg 1997;5:256–265.

    Article  PubMed  CAS  Google Scholar 

  26. Yokoyama H, Matsuoka R, Bruyere HJ, Gilbert EF, Uno H. Light and electron-microscopic observations of theophylline-induced aortic aneurysms in embryonic chicks. Am J Pathol 1983;112:258–266.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cardiovascular Surgery, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Sami Karapolat MD, Yahya Unlu MD, Bilgehan Erkut MD & Hikmet Koçak MD

  2. Department of Pathology, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Fazlı Erdoğan PhD

  3. Department of Cardiovascular Surgery, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Yahya Unlu MD

  4. Department of Cardiovascular Surgery, Faculty of Medicine, Atatürk University, Üniversite Loj. 38/8, 25240, Erzurum, Turkey

    Yahya Unlu MD

Authors
  1. Sami Karapolat MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yahya Unlu MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bilgehan Erkut MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hikmet Koçak MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fazlı Erdoğan PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yahya Unlu MD.

About this article

Cite this article

Karapolat, S., Unlu, Y., Erkut, B. et al. Influence of Indomethacin in the Rat Aneurysm Model. Ann Vasc Surg 20, 369–375 (2006). https://doi.org/10.1007/s10016-006-9023-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10016-006-9023-1

Keywords

Search

Navigation