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Immunohistochemistry on IDH 1/2, ATRX, p53 and Ki-67 substitute molecular genetic testing and predict patient prognosis in grade III adult diffuse gliomas

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Abstract

The molecular subgrouping of diffuse gliomas was recently found to stratify patients into prognostically distinct groups better than histological classification. Among several molecular parameters, the key molecules for the subtype diagnosis of diffuse gliomas are IDH mutation, 1p/19q co-deletion, and ATRX mutation; 1p/19q co-deletion is undetectable by immunohistochemistry, but is mutually exclusive with ATRX and p53 mutation in IDH mutant gliomas. Therefore, we applied ATRX and p53 immunohistochemistry instead of 1p/19q co-deletion analysis. The prognostic value of immunohistochemical diagnosis for Grade III gliomas was subsequently investigated. Then, the same immunohistochmical diagnostic approach was expanded for the evaluation of Grade II and IV diffuse glioma prognosis. The results indicate immunohistochemical analysis including IDH1/2, ATRX, p53, and Ki-67 index is valuable for the classification of diffuse gliomas, which is useful for the evaluation of prognosis, especially Grade III gliomas and lower-grade gliomas (i.e., Grade II and III).

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Acknowledgments

We thank Yoshiko Tsukada and Makiko Miyakawa for their excellent technical assistance. This work was supported in part by Grants-in-Aid for Scientific Research (to S. T., No. 15H04947) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan; and by the Japan Brain Foundation (to S. T.) and the Japanese Foundation for Multidisciplinary Treatment of Cancer (to S. T.).

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Authors and Affiliations

  1. Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan

    Shingo Takano, Eiichi Ishikawa, Noriaki Sakamoto, Masahide Matsuda, Hiroyoshi Akutsu, Tetsuya Yamamoto & Akira Matsumura

  2. Department of Pathology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan

    Noriaki Sakamoto & Masayuki Noguchi

  3. Department of Regional Innovation, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan

    Yukinari Kato

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  1. Shingo Takano
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Correspondence to Shingo Takano.

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Takano, S., Ishikawa, E., Sakamoto, N. et al. Immunohistochemistry on IDH 1/2, ATRX, p53 and Ki-67 substitute molecular genetic testing and predict patient prognosis in grade III adult diffuse gliomas. Brain Tumor Pathol 33, 107–116 (2016). https://doi.org/10.1007/s10014-016-0260-x

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