Abstract
Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7 % (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.
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Acknowledgments
This work was supported in part by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (KAKENHI Grant Number 24590406) to M.T. and (KAKENHI Grant Number 23390311) to Y.M.
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Appendix: R-IHC Study Group
Appendix: R-IHC Study Group
Shinya Tanaka, Mishie Tanino, Tomoko Takenami, Shiori Akesaka, Manami Watanabe, Eiko Aoyanagi (Hokkaido University), Akira Kurose, Emiko Mizuki, Naoya Kumagai (Hirosaki University), Yu Sugai, Noriyuki Yamada, Chikako Tomizawa (Iwate Medical University), Mareyuki Endo, Miki Aoki, Akira Morohashi, Tomoko Konta (Sendai Kousei Hospital), Kiyotaka Onodera, Manabu Suzuki, Yoshiki Kogi, Satoshi Ota, Yukio Nakatani (Chiba University), Takeo Yano, Tokuyoshi Maruyama, Tomohide Ogura, Jyunya Takeyama, Kazuki Kaneyama, Yoshiyuki Omura, Taizo Shiraishi (Mie University), Tomoo Ito, Yasuhiro Sakai, Emii Yanagida, Naoko Imagawa, Hiroshi Yamada, Tatsuko Tsukamoto (Kobe University), Shiro Takegami (Tohoku University), Satoru Kamata, Eichi Suzuki, Yoichi Akagami, Masami Kagaya, Ryuta Nakamura (Akita Industrial Technology Center), Shunsuke Wakayama, Yoshihiro Minamiya, Toshio Sasajima, Akiteru Goto, Hiroshi Nanjyo, Satoshi Ito, Hayato Konno, Yashushi Kawaharada, Shinnosuke Watanabe, Tomoaki,Yoshioka, Kasumi Narita, Naoko Takahashi, Satoshi Kudou (Akita University).
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Tanino, M., Sasajima, T., Nanjo, H. et al. Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors. Brain Tumor Pathol 32, 12–19 (2015). https://doi.org/10.1007/s10014-014-0188-y
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DOI: https://doi.org/10.1007/s10014-014-0188-y