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Fluorescence-guided resection of metastatic brain tumors using a 5-aminolevulinic acid-induced protoporphyrin IX: pathological study

Brain Tumor Pathology volume 24, pages 53–55 (2007)Cite this article

Abstract

We performed a pathological study to identify the locus of production of protoporphyrin IX (PPIX) in human metastatic brain tumors. Patients with metastatic brain tumors (n = 11) received 1 g of 5-aminolevulinic acid (5-ALA) perorally 2 h before undergoing surgery. The target region was exposed to laser light with a peak wavelength of 405 ± 1 nm and an output of 40 mW. Tissue samples from the tumor bulk and surrounding areas were examined by histological and fluorescence methods. Of the 11 tumors, 9 manifested PPIX fluorescence in the tumor bulk and peritumoral brain tissue. Our findings indicate that PPIX fluorescence can be observed in peritumoral edematous areas that are free of neoplastic cells, because PPIX produced by neoplastic cells leaks into the surrounding edematous area.

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Affiliations

  1. Department of Neurosurgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, 228-8555, Japan

    Satoshi Utsuki, Hidehiro Oka, Yoshiteru Miyajima, Satoru Shimizu, Sachio Suzuki & Kiyotaka Fujii

  2. Department of Pathology, Faculty of Medicine, University of Fukui, Fukui, Japan

    Norio Miyoshi

Authors
  1. Satoshi Utsuki
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  2. Norio Miyoshi
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  3. Hidehiro Oka
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  4. Yoshiteru Miyajima
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  5. Satoru Shimizu
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  6. Sachio Suzuki
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  7. Kiyotaka Fujii
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Corresponding author

Correspondence to Satoshi Utsuki.

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Utsuki, S., Miyoshi, N., Oka, H. et al. Fluorescence-guided resection of metastatic brain tumors using a 5-aminolevulinic acid-induced protoporphyrin IX: pathological study. Brain Tumor Pathol 24, 53–55 (2007). https://doi.org/10.1007/s10014-007-0223-3

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  • DOI: https://doi.org/10.1007/s10014-007-0223-3

Key words

  • 5-Aminolevulinic acid
  • Fluorescence diagnosis
  • Metastatic brain tumor
  • Protoporphyrin IX