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Early effects of boron neutron capture therapy on rat glioma models

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Abstract

Early effects of boron neutron capture therapy (BNCT) on malignant glioma are characterized by reduction of the enhancement area and regression of the peritumoral edema radiologically. The aim of this study was to investigate the early histological changes of tumors and inflammatory cells after BNCT in the rat brain. Rats were treated with BNCT using boronophenylalanine (BPA) 7 days after implantation of C6 glioma cells. The tumors were assessed with magnetic resonance imaging and histopathological examination at 4 days after BNCT. The mean tumor volumes were 39 ± 2 mm3 in the BNCT group and 134 ± 18 mm3 in the control group. In the BNCT group, tumor cells showed a less pleomorphic appearance with atypical nuclei and mitotic figures. The Ki-67 labeling index was 6.5% ± 4.7% in the BNCT and 35% ± 3.8% in the control group. The reactions of the inflammatory cells were examined with ED-1 as macrophage marker and OX42 as microglia marker. ED-1- and OX-42-positive cells were reduced both in the core and the marginal area of the tumor in the BNCT group. It is suggested that BNCT reduced tumor progression by suppression of proliferation. Inhibition of the activated macrophages may relate to reduced peritumoral edema in the early phase.

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Authors and Affiliations

  1. Department of Neurosurgery, Kinki University School of Medicine, 377-2 Ohnohigashi, Osakasayama, Osaka, 589-8511, Japan

    Nobuhiro Nakagawa, Fumiharu Akai, Norihito Fukawa & Mamoru Taneda

  2. Genome Biology, Kinki University School of Medicine, Osaka, Japan

    Yoshihiko Fujita

  3. Research Reactor Institute, Kyoto University, Osaka, Japan

    Minoru Suzuki & Koji Ono

Authors
  1. Nobuhiro Nakagawa
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  2. Fumiharu Akai
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  3. Norihito Fukawa
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  4. Yoshihiko Fujita
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  5. Minoru Suzuki
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  6. Koji Ono
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  7. Mamoru Taneda
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Correspondence to Nobuhiro Nakagawa.

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Nakagawa, N., Akai, F., Fukawa, N. et al. Early effects of boron neutron capture therapy on rat glioma models. Brain Tumor Pathol 24, 7–13 (2007). https://doi.org/10.1007/s10014-007-0214-4

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  • DOI: https://doi.org/10.1007/s10014-007-0214-4

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