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Effective detection of tyrosinase by Keggin-type polyoxometalate-based electrochemical sensor

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Abstract

Browning is an important factor affecting the quality of fruits and vegetables, and tyrosinase is recognized as a significant biomarker of browning. In this work, the detection effects of five Keggin-type polyoxometalates on tyrosinase were analyzed, and the detection effect of Na7PMo11NiO40 was the best. The electrochemical sensor based on Na7PMo11NiO40 was applied to the determination of tyrosinase with a rapid response (40 s), low detection limit (0.52 U·mL−1), high sensitivity (297.3 μA·mL·mg−1), wide linear detection range (3.76–27.68 U·mL−1), and high selectivity under the optimized conditions. Importantly, the applicability of the proposed sensor was explored in the detection of tyrosinase in freshly squeezed apple juice. The recoveries obtained were in the range of 98.3–101.1% (RSD < 5%). More interestingly, the molecular simulation showed more positively charged histidine residues, and van-der-Waals interactions may cause higher catalytic activity of the polyoxometalates (POMs) on tyrosinase. The active catalytic sites of POMs are mainly located in the polyacid unit rather than the transition metal ions. This work provides a promising new perspective for using polyoxometalates to detect tyrosinase in the field of clinical diagnosis, cosmetics, and food.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 21871110) and the Natural Science Foundation of Fujian Province (Grant No. 2020J01674).

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Authors and Affiliations

  1. College of Food and Biological Engineering, Jimei University, Xiamen, 361021, People’s Republic of China

    Sixue Cai, Xiaowen Chen, Li Wang & Lefang Xie

  2. School of Information Engineering, Jimei University, Xiamen, 361021, People’s Republic of China

    Jing Liu & Jiachun Zheng

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  1. Sixue Cai
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  2. Xiaowen Chen
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  3. Li Wang
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  4. Lefang Xie
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  5. Jing Liu
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Correspondence to Li Wang.

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Cai, S., Chen, X., Wang, L. et al. Effective detection of tyrosinase by Keggin-type polyoxometalate-based electrochemical sensor. J Solid State Electrochem 26, 419–429 (2022). https://doi.org/10.1007/s10008-021-05085-8

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  • DOI: https://doi.org/10.1007/s10008-021-05085-8

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