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Rapid quantitative detection of capsaicinoids in serum based on an electrochemical immunosensor with a dual-signal amplification strategy

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Abstract

In this paper, a dual-signal amplified electrochemical immunosensor for quantitative detection of capsaicinoids in serum was reported, which could be used to study the pharmacological properties of capsaicinoids. To improve the sensitivity of the immunosensor, the glassy carbon electrode was modified with Prussian blue-gold nanocomposites (PB-AuNPs) and thionine (TH). PB-AuNPs provided better electrical conductivity. At the same time, TH enhanced the stability of electron transfer, leading to superior electrochemical performance. Meanwhile, an excellent anti-capsaicinoids antibody (Ab) was used to ensure the sensitivity and specificity of the electrochemical immunosensor. The prepared immunosensor was tested using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, the proposed immunosensor displayed a wide linear range from 0.01 to 1000 ng/mL with a detection limit of 0.01 ng/mL and showed good recoveries (85.12–104.25%) and high stability. Additionally, the immunosensor was successfully applied to monitor capsaicinoids in real serum samples.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 31701681), Shandong Provincial Natural Science Foundation (ZR2017BC001), and the Major Basic Research Project of Natural Science Foundation of Shandong Province (ZR2018ZC0126).

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Authors and Affiliations

  1. School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, 255049, Shandong Province, China

    Qingxue Zhao, Xiaojie Shi, Haowei Dong, Huimin Liu, Qingqing Yang, Xia Sun & Yemin Guo

  2. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, 255049, Shandong Province, China

    Qingxue Zhao, Xiaojie Shi, Haowei Dong, Huimin Liu, Qingqing Yang, Xia Sun & Yemin Guo

  3. Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Shandong Province, 255049, Zibo, China

    Qingxue Zhao, Xiaojie Shi, Haowei Dong, Huimin Liu, Qingqing Yang, Xia Sun & Yemin Guo

  4. Shandong Lvdu Bio-Sciences & Technology Co., Ltd., Binzhou, 256600, Shandong Province, China

    Yuxiang Wu

  5. Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan, 250000, Shandong Province, China

    Yan Zheng & Shancang Zhao

Authors
  1. Qingxue Zhao
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  2. Yuxiang Wu
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  3. Xiaojie Shi
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  4. Haowei Dong
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  5. Huimin Liu
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  6. Yan Zheng
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  7. Qingqing Yang
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  8. Xia Sun
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  9. Yemin Guo
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  10. Shancang Zhao
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Correspondence to Qingqing Yang.

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Zhao, Q., Wu, Y., Shi, X. et al. Rapid quantitative detection of capsaicinoids in serum based on an electrochemical immunosensor with a dual-signal amplification strategy. J Solid State Electrochem 25, 671–681 (2021). https://doi.org/10.1007/s10008-020-04833-6

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  • DOI: https://doi.org/10.1007/s10008-020-04833-6

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