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Performance comparison with different methods for ethanol/O2 biofuel cell based on NAD+ cofactor immobilized and activated by two types of carbon nanoparticles

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Abstract

Biofuel cells are an attractive alternative to conventional fuel cells, because they use biological catalysts. We report in this article the construction of an ethanol/O2 enzymatic biofuel cell. In the first time, the performance comparison with different methods for ethanol/O2 biofuel cell has been study. Alcohol dehydrogenase, a nicotinamide adenine dinucleotide (NAD)-dependent enzyme, is immobilized with NAD+, diaphorase, and vitamin K3 (VK3) on the electrode. The oxygen is reduced at the cathode with laccase and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS). The performances of the electrodes are improved thanks to addition of carbon powder (KS6 and Super P® carbon). The benefit of the carbon particles with higher surface porosity was explained by the high-porous structure that offered a closer proximity to the reactive species and improved diffusion of ethanol and oxygen within the enzyme films. Efficiency of immobilization of NAD+ cofactor has been also demonstrated. We have shown the effect of multi-enzymatic reaction at the anode on the storage and operational stability. A high open-circuit potential has been recorded 1.02 V, demonstrating the benefit of NAD+ immobilization with maximal power density of 300 μW cm−2. Chronoamperometric measurements show a current density of 90 μA cm−2 during 20 h demonstrating the operational stability of the ethanol biofuel cell based on NAD+ cofactor immobilization.

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Acknowledgements

This work was supported by DGRSDT (MESRS) Algeria.

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Authors and Affiliations

  1. Laboratory Dynamic Interactions and Reactivity of Systems, Kasdi Merbah University, 30000, Ouargla, Algeria

    Djamel Selloum & Abdellah Henni

  2. Institut Européen des Membranes, UMR 5635, ENSCM-UMII-CNRS, Place Eugène Bataillon, 34095, Montpellier, France

    Vincent Techer, Sophie Tingry, Marc Cretin & Christophe Innocent

Authors
  1. Djamel Selloum
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  2. Vincent Techer
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  3. Abdellah Henni
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  4. Sophie Tingry
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  6. Christophe Innocent
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Correspondence to Djamel Selloum.

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Selloum, D., Techer, V., Henni, A. et al. Performance comparison with different methods for ethanol/O2 biofuel cell based on NAD+ cofactor immobilized and activated by two types of carbon nanoparticles. J Solid State Electrochem 24, 623–631 (2020). https://doi.org/10.1007/s10008-020-04506-4

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  • DOI: https://doi.org/10.1007/s10008-020-04506-4

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