Abstract
In this study, due to the oxidative and strong adhesion characteristics of mussel-inspired polydopamine, the aimed antibody-anti-CEA could be directly attached to the polydopamine-coated glassy carbon electrode surface. The immobilization method and sandwich immunoreactions strategy were applied to form a facile and effective electrochemical immunosensor. Anti-CEA was verified by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Under optimum conditions, a linear response range of CEA from 0.01 to 80 ng/mL with a low detection limit of 1.58 pg/mL was obtained by the differential pulse voltammetry determination. Assay results of clinical serum samples using the standard ELISA and reference methods gave an excellent correlation and dependence. Moreover, the preparation of immunosensor with low cost, convenient operation, and all steps processing at room temperature which possesses great potentials for practical applications.
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Funding
The work was supported by the National Natural Science Foundation of China (21604022, 21502051), the Scientific Research Fund of Hunan Provincial Education Department (15B159), the Natural Science Foundation of Hunan Province (2016JJ3097, 2018JJ3370), and the Huxiang Youth Talent Support Program (2018RS3147).
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All procedures performed in this study involving human participants were approved by The Second Xiangya Hospital, Central South University, and were in accordance with the ethical standards of Xiangya Hospital ethics committees China.
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Wang, X., Hu, X., Xiao, F. et al. A convenient signal amplification strategy for the carcinoembryonic antigen determination based on the self-polymerization of dopamine. J Solid State Electrochem 23, 2447–2453 (2019). https://doi.org/10.1007/s10008-019-04325-2
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DOI: https://doi.org/10.1007/s10008-019-04325-2