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Development of a tyrosinase-based biosensor for bisphenol A detection using gold leaf–like microstructures

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Journal of Solid State Electrochemistry Aims and scope Submit manuscript

Abstract

A simple one-step electrodeposition without template allowed the synthesis of gold microstructures on a screen-printed carbon electrode. Chloroauric ions were reduced by applying a constant potential of − 0.6 V during 600 s. A preferential growth along the <111> directions produced leaf-like structures as confirmed by scanning electron microscopy and X-ray diffraction. The as-prepared Au microstructures worked as a support for tyrosinase immobilization allowing the preparation of a highly selective and sensitive biosensor for bisphenol A (BPA) detection. The cyclic voltammograms exhibit a well-defined anodic peak at 0.24 V in phosphate buffer solution (0.1 mol L−1, pH 7.0). The enzyme creates favorable conditions for the adsorption of BPA, and after 10 min of accumulation time, the calibration curve was linear in the range of 0.5–50 μmol L−1 with a detection limit of 77 nmol L−1 (S/N = 3) and a relative standard deviation (RSD%) of 0.54% (n = 10). Furthermore, the proposed biosensor displayed long-term stability and was successfully applied to determine BPA in spiked water samples.

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Acknowledgments

The authors thanks to INCTBio, PPGQ and Propesq/UFRGS. Also, to the Center of Electron Microscopy-CME/UFRGS and CNANO/UFRGS for the facilities used for the research. Filomeno thanks to CNPQ for his Ph.D. scholarship and Manoelly O. Rocha to CAPES for her MD Scholarship.

Funding

The authors received financial support from INCTBio (CNPq/INCT 465389/2014-7), CNPQ (Process: 550441/2012-3), CNPQ (Process: 190365/2014-5), Calouste Gulbenkian Foundation. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001

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Authors and Affiliations

  1. Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

    Filomeno A. D. Inroga, Manoelly O. Rocha, Vladimir Lavayen & Jacqueline Arguello

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  1. Filomeno A. D. Inroga
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  2. Manoelly O. Rocha
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  3. Vladimir Lavayen
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  4. Jacqueline Arguello
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Correspondence to Jacqueline Arguello.

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Inroga, F.A.D., Rocha, M.O., Lavayen, V. et al. Development of a tyrosinase-based biosensor for bisphenol A detection using gold leaf–like microstructures. J Solid State Electrochem 23, 1659–1666 (2019). https://doi.org/10.1007/s10008-019-04252-2

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  • DOI: https://doi.org/10.1007/s10008-019-04252-2

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