Abstract
We report the fabrication of polyamidoamine (PAMAM) dendrimer with 128 carboxyl group-encapsulated Pt nanoparticle-modified screen-printed carbon electrode, as an impedimetric biosensor, for the quantitative detection of human cardiac biomarker troponin-I (cTnI). PAMAM-Pt was electrochemically deposited over SPCE and its 128 terminal carboxyl groups were used as anchors for the site-specific biomolecular immobilization of protein antibody, anti-cTnI. The biosensor was characterized by contact angle measurements, transmission electron microscopy, UV-visible spectroscopy, and electrochemical techniques. A single-frequency impedance analysis study was utilized for the biomolecular sensing by monitoring the changes in the phase angle obtained at an optimized frequency resulting from antigen-antibody interactions. An optimized frequency of 100 Hz was obtained at which maximum changes in the phase angle were observed after immunoreactions for a given concentration of analyte. A concentration-dependent increase in the phase angle of the biosensor was observed with increasing cTnI concentration in the range of 1 pg mL−1 to 100 ng mL−1. Based on the concentration response data, the dissociation constant was found to be 0.51 pM reflecting high affinity of biosensor towards cTnI analyte arising due to high anti-cTnI loading with a better probe orientation on the 3-dimensional PAMAM-Pt structure.
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Kitano H (2002) Systems biology: a brief overview. Science 295:1662–1664
Figeys D (2003) Proteomics in 2002: a year of technical development and wide-ranging applications. Anal Chem 75(12):2891–2905
Rajesh SV, Puri NK, Mulchandani A, Kotnala RK (2016) High performance dendrimer functionalized single-walled carbon nanotubes field effect transistor biosensor for protein detection. Appl Phys Lett 109(24):243504
Diware MS, Cho HM, Chegal W, Cho YJ, Kim DS, SW O, Kim KS, Paek SH (2017) Ultasensitive label-free detection of cardiac biomarkers with optical SIS sensors. Biosens Bioelectron 87:242–248
Lee I, Luo X, Huang J, Cui XT, Yun M (2012) Detection of cardiac biomarkers using polyaniline nanowire-based conductometric biosensors. Biosenors 2(2):205–220
Apple FS, Falahati A, Paulsen PR, Miller EA, Sharkey S (1997) Improves detection of minor ischemic myocardial injury with measurement of serum cardiac troponin I. Clin Chem 43(11):2047–2051
Kim TK, Oh SW, Hong SC, Mok YJ, Choi EY (2014) Point-of-care fluorescence immunoassay for cardiac panel biomarkers. J Clin Lab Anal 28(6):419–427
Li F, Yu Y, Cui H, Yang D, Bian Z (2013) Label free electrochemiluminescence immunosensor for cardiac troponin I using luminal functionalized gold nanoparticles as a sensing platform. Analyst 138(6):1844–1850
Wang J, Ibanez A, Chatrathi MP, Escarpa A (2001) Electrochemical enzyme immunoassays on microchip platforms. Anal Chem 73(21):5323–5327
Ho JA, Lin YC, Wang LS, Hwang KC, Chou PT (2009) Carbon nanoparticle-enhanced immunoelectrical detection for protein tumor marker with cadmium sulphide biotracers. Anal Chem 81:1340–1346
Hu C, Yang DP, Wang Z, Huang P, Wang X, Chen D, Cui D, Yang M, Jia N (2013) Bio-mimetically synthesized Ag@BSA microspheres as a novel electrochemical biosensing interface for sensitive detectionof tumor cells. Biosens Bioelectron 41:656–661
Lin D, Wu J, Wang M, Yan F, Ju H (2012) Tripal signal amplification of graphene film, polybead carried gold nanoparticles as tracing tag and silver deposition for ultrasensitive electrochemical immunosensing. Anal Chem 84(8):3662–3668
Liang G, Liu S, Zou G, Zhang X (2012) Ultrasensitive immunoassay based on anodic near-infrared electrochemiluminescence from dual-stabilizer-capped CdTe nanocrystals. Anal Chem 84(24):10645–10649
Mani V, Chikkaveeraiah BV, Patel V, Gutkind JS, Rusling JF (2009) Microfluidic electrochemical immunoassay for ultrasensitive detection of two cancer biomarker proteins in serum. ACS Nano 3(3):585–594
North SH, Lock EH, Taitt CR, Walton SG (2010) Critical aspects of biointerface design and their impact on biosensor development. Anal Bioanal Chem 397(3):925–933
Yoon HC, Kim HS (2000) Multilayered assembly of dendrimers with enzymes on gold: thickness-controlled biosensing interface. Anal Chem 72(5):922–926
Markovarga G, Johansson K, Gorton L (1964) Enzyme-based biosensor as a selective detection unit in column liquid chromatography. J Chromatogr A 660:153–167
Boujtita M, Chapleau M, El Murr N (1996) Biosensors for analysis of ethanol in food: effect of the pasting liquid. Anal Chim Acta 319(1-2):91–96
Hasanzadeh H, Shadzou N, Eskandani M, Soleymani J, Jafari F, Guardia M (2014) Dendrimer-encapsulated and cored metal nanoparticles for electrical nanobiosensing. TrAC 53:137–149
Bas SZ, Gulce H, Yildiz S, Gulce A (2011) Amperometric biosensors based on deposition of gold and platinum nanoparticles on polyvinylferrocene modified electrode for xanthine detection. Talanta 87:189–196
Rusmini F, Zhong Z, Feijen J (2007) Protein immobilization strategies for protein biochips. Biomacromolecules 8:1755–1789
Negahdary M, Ardakani MB, Sattarahmady N, Yadegari H, Heli H (2017) Electrochemical aptasensing of human cardiac troponin I based on an array of gold nanodumbbells-applied to early detection of myocardial infarction. Sensors Actuators B: Chem 252:62–71
Shanmugam NR, Muthukumar S, Chaudhry S, Anguiano J (2017) Prasad S. Biosens Bioelectron. https://doi.org/10.1016/j.bios.2016.10.046
Yu T, Wang W, Chen J, Zeng Y, Li Y, Yang G, Li Y (2012) Dendrimer-encapsulated Pt nanoparticles: an artificial enzyme for hydrogen production. J Phys Chem C 116(19):10516–10521
Zhao M, Crooks RM (1999) Angew Chem Int Ed 38:364
Liu DX, Gao JX, Murphy CJ, Williams CT (2004) In situ attenuated total reflection infrared spectroscopy of dendrimer-stabilized Pt nanoparticles adsorbed on alumina. J Phys Chem B 108:12911
Liu C, Zhang H, Tang Y, Luo S (2014) Controllable growth of graphene/Cu composite and its nanoarchitecture-dependent electrocatalytic activity to hydrazine oxidation. J Mater Chem A 2(13):4580–4587
Arotiba OA, Owino JH, Baker PG, Iwuoha EI (2010) Electrochemical impedimetry of electrodeposited poly (propylene imine) dendrimer monolayer. J Electroanal Chem 638(2):287–292
Monk PMS (2005) Fundamentals of electroanalytical chemistry. Wiley, England
Ko S, Kim B, Jo SS, Oh SY, Park JK (2007) Electrochemical detection of cardiac troponin I using a microchip with the surface-functionalized poly (dimethylsiloxane) channel. Biosens Bioelectron 23(1):51–59
Periyakaruppan A, Gandhiraman RP, Meyyappan M, Koehne JE (2013) Label-free detection of cardiac troponin-I using carbon nanofibre based nanoelectrode array. Anal Chem 85(8):3858–3863
Li Z, Ma KE, Cheng Z, Yan C, Liu G (2017) Fabrication of electrochemical immunosensor for cardiac biomarker troponin I determination and its potential for acute myocardial infarction diagnosis. Int J Electrochem Sci 12:2389–2399
AAhammad AJS, Choi YH, Koh K, Kim JH, Lee JJ, Lee M (2011) Electrochemical detection of cardiac biomarker troponin I at gold nanoparticle-modified ITO electrode by using open circuit potential. Int J Electrochim Sci 6:1906
Shumkov AA, Suprun EV, Shatinina SZ, Lisitsa AV, Shumyantseva VV, Archakov AI (2013) Gold and silver nanoparticles for electrochemical detection of cardiac troponin I based on stripping voltammetr. J Bionanosci 3(2):216–222
Shu-Hai J, Ting F, Juan LL, Yi C, Qing ZX, Liang SZ, Liang LY, Kun SZ (2014) The detection of cTnI by the aptamer biosensor. Prog Biochem Biophys 41:916–920
Kumar S, Kumar S, Augustine S, Malhotra BD (2017) Protein functionalized nanostructured zirconia based electrochemical immunosensor for cardiac troponin I detection. J. Mater. https://doi.org/10.1557/jmr.2017.102
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Singal, S., Srivastava, A.K., Kotnala, R.K. et al. Single-frequency impedance analysis of biofunctionalized dendrimer-encapsulated Pt nanoparticles-modified screen-printed electrode for biomolecular detection. J Solid State Electrochem 22, 2649–2657 (2018). https://doi.org/10.1007/s10008-018-3977-0
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DOI: https://doi.org/10.1007/s10008-018-3977-0