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Surfactant-assisted preparation of nanohybrid for simultaneously improving enzyme-immobilization and electron-transfer in biosensor and biofuel cell

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Abstract

As enzyme-immobilization and electron-transfer are the key factors for fabricating an enzymatic bioelectrode and its devices, we investigated a strategy to simultaneously improve the two aspects by assistance of the cationic surfactant, stearyltrimethylammonium bromide (STAB). By electrodeposition method, we obtained a multifunctional STAB-modified nanomaterial on electrode surface to improve enzyme-immobilization and electron-transfer. On the one hand, STAB could firmly adsorb a substantial number of enzymes via electrostatic interaction in a favorable orientation on the conductive nanomaterial surface for electron-transfer. On the other hand, STAB acts as a dispersant and stabilizer for traditionally conductive nanomaterials (reduced graphene oxide, carbon nanotubes, and gold nanoparticles) to guarantee their unique properties and form a well-conductive network. Electrochemical measurements demonstrated that enzymatic electrodes based on the nanohybrid possessed fast electron-transfer rate, a large quantity of immobilized enzymes, and good activity toward glucose oxidation or oxygen reduction. The glucose biosensor performed linear response range of 0.01–11.71 mM, detection limit of 3.84 × 10−3 mM, and sensitivity of 10.42 μA mM−1 cm−2, while the glucose/O2 biofuel cell exhibited maximum power density of 121.87 μW cm−2 and open-circuit voltage of 0.663 V. Both of the devices showed better performances than those of devices without STAB or conductive nanomaterials in this work.

Fabrication of surfactant-modified enzymatic electrode and its electrochemical performance

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Acknowledgments

This research is supported by the National Natural Science Foundation of China (Grant 51372206).

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Authors and Affiliations

  1. Department of Applied Chemistry, School of Natural and Applied Sciences, Northwestern Polytechnical University, Xi’an, 710129, People’s Republic of China

    Fengjin Qu, Xiaoyan Ma, Yuchen Hui, Fang Chen, Yan Gao & Ying Chen

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  1. Fengjin Qu
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  2. Xiaoyan Ma
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  3. Yuchen Hui
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Correspondence to Xiaoyan Ma.

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Qu, F., Ma, X., Hui, Y. et al. Surfactant-assisted preparation of nanohybrid for simultaneously improving enzyme-immobilization and electron-transfer in biosensor and biofuel cell. J Solid State Electrochem 21, 1545–1557 (2017). https://doi.org/10.1007/s10008-017-3509-3

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  • DOI: https://doi.org/10.1007/s10008-017-3509-3

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