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Electrochemical sensor for determination of aflatoxin B1 based on multiwalled carbon nanotubes-supported Au/Pt bimetallic nanoparticles

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Abstract

A sensitive and selective imprinted electrochemical sensor for the determination of aflatoxin B1 (AFB1) was constructed on a glassy carbon electrode by stepwise modification of functional multiwalled carbon nanotubes (MCNTs), Au/Pt bimetallic nanoparticles (Au/PtNPs), and a thin imprinted film. The fabrication of a homogeneous porous poly o-phenylenediamine (POPD)-grafted Au/Pt bimetallic multiwalled carbon nanotubes nanocomposite film was conducted by controllable electrodepositing technology. The sensitivity of the sensor was improved greatly because of the nanocomposite functional layer; the proposed sensor exhibited excellent selectivity toward AFB1 owing to the porous molecular imprinted polymer (MIP) film. The surface morphologies of the modified electrodes were characterized using a scanning electron microscope. The performance of the imprinted sensor was investigated by cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy in detail. A linear relationship between the sensor response signal and the logarithm of AFB1 concentrations ranging from 1 × 10−10 to 1 × 10−5 mol L−1 was obtained with a detection limit of 0.03 nmol L−1. It was applied to detect AFB1 in hogwash oil successfully.

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References

  1. Alexander C, Andersson HS, Andersson LI, Ansell RJ, Kirsch N, Nicholls IA, O’Mahony J, Whitcombe MJ (2006) Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003. J Mol Recognit 19:106–180

    Article  CAS  Google Scholar 

  2. Wulff G, Sarhan A (1972) Use of polymers with enzyme-analogous structures for the resolution of racemates. Angew Chem Int Ed Engl 11:341–344

    CAS  Google Scholar 

  3. Lahav M, Katz E, Willner I (2001) Photochemical imprint of molecular recognition sites in two-dimensional monolayers assembled on Au electrodes: effects of the monolayer structures on the binding affinities and association kinetics to the imprinted interfaces. Langmuir 17:7387–7395

    Article  CAS  Google Scholar 

  4. Yang HH, Zhang SQ, Yang W, Chen XL, Zhuang ZX, Xu JG, Wang XR (2004) Molecularly imprinted sol-gel nanotubes membrane for biochemical separations. J Am Chem Soc 126:4054–4055

    Article  CAS  Google Scholar 

  5. Lübke M, Whitcombe MJ, Vulfson EN (1998) A novel approach to the molecular imprinting of polychlorinated aromatic compounds. J Am Chem Soc 120:13342–13348

    Article  Google Scholar 

  6. Xie C, Li H, Li S, Wu J, Zhang Z (2009) Surface molecular self-assembly for organophosphate pesticide imprinting in electropolymerized poly(p-aminothiophenol) membranes on a gold nanoparticle modified glassy carbon electrode. Anal Chem 82:241–249

    Article  Google Scholar 

  7. Apodaca DC, Pernites RB, Ponnapati R, Del Mundo FR, Advincula RC (2011) Electropolymerized molecularly imprinted polymer film: EIS sensing of bisphenol A. Macromolecules 44:6669–6682

    Article  CAS  Google Scholar 

  8. Riskin M, Tel-Vered R, Bourenko T, Granot E, Willner I (2008) Imprinting of molecular recognition sites through Elec Vered tropolymerization of functionalized Au nanoparticles: development of an electrochemical TNT sensor based on π-donor-acceptor interactions. J Am Chem Soc 130:9726–9733

    Article  CAS  Google Scholar 

  9. Pietrzyk A, Kutner W, Chitta R, Zandler ME, D’Souza F, Sannicolò F, Mussini PR (2009) Melamine acoustic chemosensor based on molecularly imprinted polymer film. Anal Chem 81:10061–10070

    Article  CAS  Google Scholar 

  10. Panasyuk TL, Mirsky VM, Piletsky SA, Wolfbeis OS (1999) Electropolymerized molecularly imprinted polymers as receptor layers in capacitive chemical sensors. Anal Chem 71:4609–4613

    Article  CAS  Google Scholar 

  11. Haupt K, Mosbach K (2000) Molecularly imprinted polymers and their use in biomimetic sensors. Chem Rev 100:2495–2504

    Article  CAS  Google Scholar 

  12. Li J, Li S, Wei X, Tao H, Pan H (2012) Molecularly imprinted electrochemical luminescence sensor based on signal amplification for selective determination of trace Gibberellin A3. Anal Chem 84:9951–9955

    Article  CAS  Google Scholar 

  13. Hu Y, Zhang Z, Zhang H, Luo L, Yao S (2012) Selective and sensitive molecularly imprinted sol-gel film-based electrochemical sensor combining mercaptoacetic acid-modified PbS nanoparticles with Fe3O4@Au–multi-walled carbon nanotubes-chitosan. J Solid State Electrochem 16:857–867

    Article  CAS  Google Scholar 

  14. Song W, Chen Y, Xu J, Yang X, Tian D (2010) Dopamine sensor based on molecularly imprinted electrosynthesized polymers. J Solid State Electrochem 14:1909–1914

    Article  CAS  Google Scholar 

  15. Meng L, Qiao X, Song J, Xu Z, Xin J, Zhang Y (2011) Study of an online molecularly imprinted solid phase extraction coupled to chemiluminescence sensor for the determination of trichlorfon in vegetables. J Agric Food Chem 59:12745–12751

    Article  CAS  Google Scholar 

  16. Turiel E, Tadeo JL, Martin-Esteban A (2007) Molecularly imprinted polymeric fibers for solid-phase microextraction. Anal Chem 79:3099–3104

    Article  CAS  Google Scholar 

  17. Masqué N, Marcé RM, Borrull F, Cormack PAG, Sherrington DC (2000) Synthesis and evaluation of a molecularly imprinted polymer for selective on-line solid-phase extraction of 4-Nitrophenol from Environmental Water. Anal Chem 72:4122–4126

    Article  Google Scholar 

  18. Koster EHM, Crescenzi C, den Hoedt W, Ensing K, de Jong GJ (2001) Fibers coated with molecularly imprinted polymers for solid-phase microextraction. Anal Chem 73:3140–3145

    Article  CAS  Google Scholar 

  19. Schweitz L, Andersson LI, Nilsson S (1997) Capillary electrochromatography with predetermined selectivity obtained through molecular imprinting. Anal Chem 69:1179–1183

    Article  CAS  Google Scholar 

  20. Priego-Capote F, Ye L, Shakil S, Shamsi SA, Nilsson S (2008) Monoclonal behavior of molecularly imprinted polymer nanoparticles in capillary electrochromatography. Anal Chem 80:2881–2887

    Article  CAS  Google Scholar 

  21. Schweitz L (2002) Molecularly imprinted polymer coatings for open-tubular capillary electrochromatography prepared by surface initiation. Anal Chem 74:1192–1196

    Article  CAS  Google Scholar 

  22. Ou J, Li X, Feng S, Dong J, Dong X, Kong L, Ye M, Zou H (2006) Preparation and evaluation of a molecularly imprinted polymer derivatized silica monolithic column for capillary electrochromatography and capillary liquid chromatography. Anal Chem 79:639–646

    Article  Google Scholar 

  23. Das K, Penelle J, Rotello VM (2003) Selective picomolar detection of hexachlorobenzene in water using a quartz crystal microbalance coated with a molecularly imprinted polymer thin film. Langmuir 19:3921–3925

    Article  CAS  Google Scholar 

  24. Lee MH, Thomas JL, Tseng HY, Lin WC, Liu BD, Lin HY (2011) Sensing of digestive proteins in saliva with a molecularly imprinted poly (ethylene-co-vinyl alcohol) thin film coated quartz crystal microbalance sensor. ACS Appl Mater Interfaces 3:3064–3071

    Article  CAS  Google Scholar 

  25. Apodaca DC, Pernites RB, Ponnapati RR, Del Mundo FR, Advincula RC (2010) Electropolymerized molecularly imprinted polymer films of a bis-terthiophene dendron: folic acid quartz crystal microbalance sensing. ACS Appl Mater Interfaces 3:191–203

    Article  Google Scholar 

  26. Fu Y, Finklea HO (2003) Quartz crystal microbalance sensor for organic vapor detection based on molecularly imprinted polymers. Anal Chem 75:5387–5393

    Article  CAS  Google Scholar 

  27. Ersöz A, Diltemiz SE, Özcan AA, Denizli A, Say R (2008) Synergie between molecular imprinted polymer based on solid-phase extraction and quartz crystal microbalance technique for 8-OHdG sensing. Biosens Bioelectron 24:742–747

    Article  Google Scholar 

  28. Byun HS, Youn YN, Yun YH, Yoon SD (2010) Selective separation of aspirin using molecularly imprinted polymers. Sep Purif Technol 74:144–153

    Article  CAS  Google Scholar 

  29. Wang HJ, Zhou WH, Yin XF, Zhuang ZX, Yang HH, Wang XR (2006) Template synthesized molecularly imprinted polymer nanotube membranes for chemical separations. J Am Chem Soc 128:15954–15955

    Article  CAS  Google Scholar 

  30. Ye L, Ramström O, Mosbach K (1998) Molecularly imprinted polymeric adsorbents for byproduct removal. Anal Chem 70:2789–2795

    Article  CAS  Google Scholar 

  31. Wu B, Hu D, Kuang Y, Yu Y, Zhang X, Chen J (2011) High dispersion of platinum-ruthenium nanoparticles on the 3,4,9,10-perylene tetracarboxylic acid-functionalized carbon nanotubes for methanol electro-oxidation. Chem Commun 47:5253–5255

    Article  CAS  Google Scholar 

  32. Che G, Lakshmi BB, Martin CR, Fisher ER (1999) Metal-nanocluster-filled carbon nanotubes: catalytic properties and possible applications in electrochemical energy storage and production. Langmuir 15:750–758

    Article  CAS  Google Scholar 

  33. Tsai Y, Hong Y (2008) Electrochemical deposition of platinum nanoparticles in multiwalled carbon nanotube-Nafion composite for methanol electrooxidation. J Solid State Electrochem 12:1293–1299

    Article  CAS  Google Scholar 

  34. Wang Z, Zhao D, Zhao G, Li H (2009) Ultrasonic assisted polyol synthesis of highly dispersed Pt/MWCNT electrocatalyst for methanol oxidation. J Solid State Electrochem 13:371–376

    Article  CAS  Google Scholar 

  35. Jin G, Chen L, Hang G, Yang S, Wu X (2010) Stripping chronopotentiometric analysis of cysteine on nano-silver coat polyquercetin-MWCNT modified platinum electrode. J Solid State Electrochem 14:1163–1169

    Article  CAS  Google Scholar 

  36. Shahrokhian S, Hosseini-Nassab N, Kamalzadeh Z (2014) Fabrication of an electrochemical sensor based on the electrodeposition of Pt nanoparticles on multiwalled carbon nanotubes film for voltammetric determination of ceftriaxone in the presence of lidocaine, assisted by factorial-based response-surface methodology. J Solid State Electrochem 18:77–88

    Article  CAS  Google Scholar 

  37. Mani V, Wu T, Chen S (2014) Iron nanoparticles decorated graphene-multiwalled carbon nanotubes nanocomposite-modified glassy carbon electrode for the sensitive determination of nitrite. J Solid State Electrochem 18:1015–1023

    Article  CAS  Google Scholar 

  38. Venkateswara Rao C, Cabrera CR, Ishikawa Y (2011) Graphene-supported Pt-Au alloy nanoparticles: a highly efficient anode for direct formic acid fuel cells. J Phys Chem C 115:21963–21970

    Article  CAS  Google Scholar 

  39. Jin G, Peng X, Ding Y, Liu W, Ye J (2009) Electrodeposition of platinum-nickel alloy nanocomposites on polyaniline-multiwalled carbon nanotubes for carbon monoxide redox. J Solid State Electrochem 13:967–973

    Article  CAS  Google Scholar 

  40. Wang L, Yamauchi Y (2010) Autoprogrammed synthesis of triple-layered Au@Pd@Pt core-shell nanoparticles consisting of a Au@Pd bimetallic core and nanoporous Pt shell. J Am Chem Soc 132:13636–13638

    Article  CAS  Google Scholar 

  41. Wang L, Yamauchi Y (2011) Strategic synthesis of trimetallic Au@Pd@Pt core-shell nanoparticles from poly(vinylpyrrolidone)-based aqueous solution toward highly active electrocatalysts. Chem Mater 23:2457–2465

    Article  CAS  Google Scholar 

  42. Zeng J, Yang J, Lee JY, Zhou W (2006) Preparation of carbon-supported core-shell Au-Pt nanoparticles for methanol oxidation reaction: the promotional effect of the Au Core. J Phys Chem B 110:24606–24611

    CAS  Google Scholar 

  43. Lee YW, Kim NH, Lee KY, Kwon K, Kim M, Han SW (2008) Synthesis and characterization of flower-shaped porous Au−Pd alloy nanoparticles. J Phys Chem C 112:6717–6722

    Article  CAS  Google Scholar 

  44. Garcia-Gutierrez DI, Gutierrez-Wing CE, Giovanetti L, Ramallo-López JM, Requejo FG, Jose-Yacaman M (2005) Temperature effect on the synthesis of Au−Pt bimetallic nanoparticles. J Phys Chem B 109:3813–3821

    CAS  Google Scholar 

  45. Wu ML, Chen DH, Huang TC (2001) Preparation of Au/Pt bimetallic nanoparticles in water-in-oil microemulsions. Chem Mater 13:599–606

    Article  CAS  Google Scholar 

  46. Deng L, Hu W, Deng H, Xiao S (2010) Surface segregation and structural features of bimetallic Au−Pt nanoparticles. J Phys Chem C 114:11026–11032

    Article  CAS  Google Scholar 

  47. Park JB, Conner SF, Chen DA (2008) Bimetallic Pt−Au clusters on TiO2(110): growth, surface composition, and metal-support interactions. J Phys Chem C 112:5490–5500

    Article  CAS  Google Scholar 

  48. Long NV, Chien ND, Uchida M, Matsubara T, Randy J, Masayuki N (2010) Directed and random self-assembly of Pt-Au nanoparticles. Mater Chem Phys 124:1193–1197

    CAS  Google Scholar 

  49. Wilson AS, Williams DP, Davis CD, Tingle MD, Park BK (1997) Bioactivation and inactivation of aflatoxin B1 by human, mouse and rat liver preparations: Effect on SCE in human mononuclear leucocytes. Mutat Res Fundam Mol Mech Mutagen 373:257–264

    Article  CAS  Google Scholar 

  50. Diao E, Shan C, Hou H, Wang S, Li M, Dong H (2012) Structures of the ozonolysis products and ozonolysis pathway of aflatoxin B1 in acetonitrile solution. J Agric Food Chem 60:9364–9370

    Article  CAS  Google Scholar 

  51. Blesa J, Soriano JM, Moltó JC, Marín R, Mañes J (2003) Determination of aflatoxins in peanuts by matrix solid-phase dispersion and liquid chromatography. J Chromatogr A 1011:49–54

    Article  CAS  Google Scholar 

  52. Stroka J, Anklam E (2002) New strategies for the screening and determination of aflatoxins and the detection of aflatoxin-producing moulds in food and feed. TrAC Trends Anal Chem 21:90–95

    Article  CAS  Google Scholar 

  53. Park JW, Kim YB (2006) Effect of pressure cooking on aflatoxin B1 in rice. J Agric Food Chem 54:2431–2435

    Article  CAS  Google Scholar 

  54. Xiong K, Liu HJ, Li LT (2012) Product identification and safety evaluation of aflatoxin B1 decontaminated by electrolyzed oxidizing water. J Agric Food Chem 60:9770–9778

    Article  CAS  Google Scholar 

  55. Raney KD, Gopalakrishnan S, Byrd S, Stone MP, Harris TM (1990) Alteration of the aflatoxin cyclopentenone ring to a delta-lactone reduces intercalation with DNA and decreases formation of guanine N7 adducts by aflatoxin epoxides. Chem Res Toxicol 3:254–261

    Article  CAS  Google Scholar 

  56. Hashemi J, Kram GA, Alizadeh N (2008) Enhanced spectrofluorimetric determination of aflatoxin B1 in wheat by second-order standard addition method. Talanta 75:1075–1081

    Article  CAS  Google Scholar 

  57. Ardic M, Karakaya Y, Atasever M, Durmaz H (2008) Determination of aflatoxin B1 levels in deep-red ground pepper (isot) using immunoaffinity column combined with ELISA. Food Chem Toxicol 46:1596–1599

    Article  CAS  Google Scholar 

  58. Holcomb M, Thompson HC Jr, Cooper WM, Hopper ML (1996) SFE extraction of aflatoxins (B1, B2, G1, and G2) from corn and analysis by HPLC. J Supercrit Fluids 9:118–121

    Article  CAS  Google Scholar 

  59. Jaimez J, Fente CA, Vazquez BI, Franco CM, Cepeda A, Mahuzier G, Prognon P (2000) Application of the assay of aflatoxins by liquid chromatography with fluorescence detection in food analysis. J Chromatogr A 882:1–10

    Article  CAS  Google Scholar 

  60. Adányi N, Levkovets IA, Rodriguez-Gil S, Ronald A, Váradi M, Szendrő I (2007) Development of immunosensor based on OWLS technique for determining Aflatoxin B1 and Ochratoxin A. Biosens Bioelectron 22:797–802

    Article  Google Scholar 

  61. Wu B, Wang Z, Xue Z, Zhou X, Du J, Liu X, Lu X (2012) A novel molecularly imprinted electrochemiluminescence sensor for isoniazid detection. Analyst 137:3644–3652

    Article  CAS  Google Scholar 

  62. Ballarin B, Gazzano M, Scavetta E, Tonelli D (2009) One-step electrosynthesis of bimetallic Au-Pt nanoparticles on indium tin oxide electrodes: effect of the deposition parameters. J Phys Chem C 113:15148–15154

    Article  CAS  Google Scholar 

  63. Lahav M, Katz E, Doron A, Patolsky F, Willner I (1999) Photochemical imprint of molecular recognition sites in monolayers assembled on Au electrodes. J Am Chem Soc 121:862–863

    Article  CAS  Google Scholar 

  64. Li J, Jiang F, Wei X (2010) Molecularly imprinted sensor based on an enzyme amplifier for ultratrace oxytetracycline determination. Anal Chem 82:6074–6078

    Article  CAS  Google Scholar 

  65. Tan CJ, Chua HG, Ker KH, Tong YW (2008) Preparation of bovine serum albumin surface-imprinted submicrometer particles with magnetic susceptibility through core−shell miniemulsion polymerization. Anal Chem 80:683–692

    Article  CAS  Google Scholar 

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (No. 20965007). We also thank Key Laboratory of Eco-Environment-Related Polymer Materials (Northwest Normal University), Ministry of Education, for the financial support.

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  1. Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, People’s Republic of China

    Zhihua Wang, Jinshu Li, Lijuan Xu, Yanjun Feng & Xiaoquan Lu

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  1. Zhihua Wang
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  2. Jinshu Li
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  3. Lijuan Xu
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  5. Xiaoquan Lu
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Correspondence to Zhihua Wang.

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Wang, Z., Li, J., Xu, L. et al. Electrochemical sensor for determination of aflatoxin B1 based on multiwalled carbon nanotubes-supported Au/Pt bimetallic nanoparticles. J Solid State Electrochem 18, 2487–2496 (2014). https://doi.org/10.1007/s10008-014-2506-z

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  • DOI: https://doi.org/10.1007/s10008-014-2506-z

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