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Amperometric biosensor based on tyrosinase immobilized in hydrotalcite-like compounds film for the determination of polyphenols

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Abstract

A tyrosinase (Tyr) biosensor has been constructed by immobilizing tyrosinase on the surface of Mg–Al–CO3 hydrotalcite-like compound film (HTLc) modified glassy carbon electrode (GCE) for the determination of polyphenols. The negatively charged tyrosinase was adsorbed firmly on the surface of a positively charged HTLc/GCE by electrostatic interactions and retained its activity to a great degree. The modified electrode was characterized by cyclic voltammetry and AC impedance spectra. Polyphenols were determined by a direct reduction of biocatalytically generated quinone species. The different parameters, including pH, temperature, and enzyme loading were investigated and optimized. Under the optimum conditions, Tyr/HTLc electrode gave a linear response range of 3–300, 0.888–444, and 0.066–396 μM with a detection limit (S/N = 3) of 0.1, 0.05, and 0.003 μM for catechol, caffeic acid, and quercetin, respectively. In addition, the repeatability and stability of the enzyme electrode were estimated. Total polyphenol contents of real samples were also determined to study the potential applicability of the Tyr/HTLc/GCE biosensor.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 21075078).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Tai’an, 271018, Shandong, People’s Republic of China

    Ruixia Han, Lin Cui, Shiyun Ai, Huanshun Yin, Xianggang Liu & Yanyan Qiu

  2. College of Resources and Environment, Shandong Agricultural University, Tai’an, 271018, Shandong, People’s Republic of China

    Huanshun Yin

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  1. Ruixia Han
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  2. Lin Cui
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Correspondence to Shiyun Ai.

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Han, R., Cui, L., Ai, S. et al. Amperometric biosensor based on tyrosinase immobilized in hydrotalcite-like compounds film for the determination of polyphenols. J Solid State Electrochem 16, 449–456 (2012). https://doi.org/10.1007/s10008-011-1352-5

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  • DOI: https://doi.org/10.1007/s10008-011-1352-5

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