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An amperometric urea biosensor based on covalent immobilization of urease on copolymer of glycidyl methacrylate and vinylferrocene

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Abstract

A unique urea biosensor construction based on the direct covalent attachment of urease onto a polymeric electron transfer mediator, poly(glycidyl methacrylate-co-vinylferrocene)-coated electrode is described. Amperometric response was measured as a function of urea concentration, at a fixed potential of +0.35 V vs. Ag/AgCl in phosphate-buffered saline (pH 7.0). Covalent immobilization of the urease directly to the functionalized ferrocene copolymer surface produced biosensors with a short response time (about 3 s) and provided low detection limits. The stability, reusability, pH, and temperature response of the biosensor, besides its kinetic parameter, were also studied.

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References

  1. Aronson D (2004) Am J Med 116:466

    Article  CAS  Google Scholar 

  2. Ronco C, Bellomo R, Homel P, Brendolan A, Dan M, Piccinni P, Greca GL (2000) Lancet 355:26

    Article  Google Scholar 

  3. Dhawan G, Sumana G, Malhotra BD (2009) Biochem Eng J 44:42

    Article  CAS  Google Scholar 

  4. Singhal R, Gambhir A, Pandey MK, Annapoorni S, Malhotra BD (2002) Biosens Bioelectron 17:697

    Article  CAS  Google Scholar 

  5. Melo JVD, Cosnier S, Mousty C, Martelet C, Renault NJ (2002) Anal Chem 74:4037

    Article  Google Scholar 

  6. Cho WJ, Huang HJ (1998) Anal Chem 70:3946

    Article  CAS  Google Scholar 

  7. Maaref A, Barhoumi H, Rammah M, Martelet C, Jaffrezic-Renault N, Mousty C, Cosnier S (2007) Sens Actuators B Chem 123:671

    Article  Google Scholar 

  8. Vostiar I, Tkac J, Sturdik E, Gemeiner P (2002) Bioelectrochemistry 56:113

    Article  CAS  Google Scholar 

  9. Pizzariello A, Stredansky M, Stredanska S, Miertus S (2000) Talanta 54:763

    Article  Google Scholar 

  10. Stredansky M, Pizzariello A, Stredanska S, Miertus S (2000) Anal Chim Acta 415:151

    Article  CAS  Google Scholar 

  11. Saito T, Watanabe M (1998) React Funct Polym 37:263

    Article  CAS  Google Scholar 

  12. Koide S, Yokoyama K (1999) J Electroanal Chem 468:193

    Article  CAS  Google Scholar 

  13. Kuramoto N, Shishido Y, Nagai K (1994) Macromol Rapid Commun 15:441

    Article  CAS  Google Scholar 

  14. Kandimalla VB, Tripathi VS, Ju H (2006) Biomaterials 27:1167

    Article  CAS  Google Scholar 

  15. Akgöl S, Kacar Y, Denizli A, Arıca MY (2001) Food Chem 74:281

    Article  Google Scholar 

  16. Şenel M, Çevik E, Abasıyanık MF (2010) Sens Actuators B 145:444

    Article  Google Scholar 

  17. Spagna G, Barbagallo RN, Pifferi PG, Blanco RM, Guisan JM (2000) J Mol Catal B Enzym 11:63

    Article  CAS  Google Scholar 

  18. Nanjundan S, Unnithan CS, Selvamalar CSJ, Penlidis A (2005) React Funct Polym 62:11

    Article  Google Scholar 

  19. Robinson KL, Lawrence NS (2008) Anal Sci 24:339

    Article  CAS  Google Scholar 

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Acknowledgment

This research was supported by grants from T.R. Prime Ministry State Planning Organization and Fatih University Research Support Office (P50090801-1).

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul, 34500, Turkey

    Emre Çevik & Mustafa Fatih Abasıyanık

  2. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul, 34500, Turkey

    Mehmet Şenel

  3. Department of Chemistry, Fatih University, Buyukcekmece Kampusu, 34500, B.Cekmece, Istanbul, Turkey

    Mehmet Şenel

Authors
  1. Emre Çevik
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  2. Mehmet Şenel
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  3. Mustafa Fatih Abasıyanık
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Correspondence to Mehmet Şenel.

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Çevik, E., Şenel, M. & Abasıyanık, M.F. An amperometric urea biosensor based on covalent immobilization of urease on copolymer of glycidyl methacrylate and vinylferrocene. J Solid State Electrochem 16, 367–373 (2012). https://doi.org/10.1007/s10008-011-1335-6

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  • DOI: https://doi.org/10.1007/s10008-011-1335-6

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