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Electrochemiluminescence DNA sensor based on Ru(bpy) 2+3 -doped silica nanoparticle labeling and proximity-dependent surface hybridization assay

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Abstract

A new electrochemiluminescence (ECL) method based on the proximity-dependent surface hybridization assay and Ru(bpy) 2+3 -doped silica nanoparticles (Ru-DSNPs) as labels were proposed for detecting DNA. The hybridization process involves two steps: firstly, the 3′ thiolated capture probe was self-assembled on the gold electrode. Secondly, the proximity-dependent surface hybridization assay was carried out. This proximity-dependent surface hybridization assay depended on the simultaneous recognition of a target DNA by a capture probe and Ru-DSNP-labeled probe and the formation of a duplex complex, which results in the luminophor approach to the electrode surface. Thus, sensitive ECL signals were obtained. Under optimum conditions, the intensity of the ECL of Ru-DSNPs was linearly related to the concentration of the target sequence in the range of 2.0 × 10−15 to 2.0 × 10−11 mol/L. The detection limit was 1.0 × 10−15 mol/L (S/N = 3).

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (grant No. 20975061), National Basic Research Program of China (grant no. 2007CB936602), and Natural Science Foundation of Shandong Province in China (grant no. Y2008B20).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People’s Republic of China

    Qixia Sun & Xiaoli Zhang

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  1. Qixia Sun
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  2. Xiaoli Zhang
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Correspondence to Xiaoli Zhang.

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Sun, Q., Zhang, X. Electrochemiluminescence DNA sensor based on Ru(bpy) 2+3 -doped silica nanoparticle labeling and proximity-dependent surface hybridization assay. J Solid State Electrochem 16, 247–252 (2012). https://doi.org/10.1007/s10008-011-1321-z

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  • DOI: https://doi.org/10.1007/s10008-011-1321-z

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