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Carbon nanotubes/(pLys/dsDNA) n layer-by-layer multilayer films for electrochemical studies of DNA damage

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Abstract

Carboxylic group-functionalized carbon nanotubes (c-CNT) were modified on the surface of carbon paste electrode to obtain a conducting precursor film. Positively charged poly-l-lysine (pLys) and negatively charged double-stranded DNA (dsDNA) were alternately adsorbed on the c-CNT-modified electrode, forming (pLys/dsDNA) n layer-by-layer (LBL) films. Cyclic voltammetry and electrochemical impedance spectroscopy of the electroactive probe [Fe(CN)6]3−/4− could give the valuable dynamic information of multilayer films growth. The oxidative DNA damage induced by cadmium ion (Cd2+) in the LBL multilayer films was studied by differential pulse voltammetry (DPV) with methylene violet (MV) as the intercalation redox probe. The electrochemical signals of MV on the multilayer films were effectively amplified via LBL technology. The specific intercalation of MV into dsDNA base pairs and the amplified electrochemical response of MV, combined with the unique feature of loading reversibility of MV in the DNA layer-by-layer films, made the difference in DPV response between the intact, and damaged dsDNA films become pronounced. This biosensor exhibited that the (pLys/dsDNA) n films could be utilized for investigations of DNA damage.

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (nos. 20635020, 20805025, and 20975057), Doctoral Foundation of the Ministry of Education of China (no. 20060426001), and the Natural Science Foundation of Qingdao City (no. 09-1-3-25-jch).

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Authors and Affiliations

  1. Key Laboratory of Eco-chemical Engineering (Ministry of Education), College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Meng Du, Tao Yang & Kui Jiao

Authors
  1. Meng Du
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  2. Tao Yang
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  3. Kui Jiao
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Correspondence to Kui Jiao.

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Du, M., Yang, T. & Jiao, K. Carbon nanotubes/(pLys/dsDNA) n layer-by-layer multilayer films for electrochemical studies of DNA damage. J Solid State Electrochem 14, 2261–2266 (2010). https://doi.org/10.1007/s10008-010-1059-z

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  • DOI: https://doi.org/10.1007/s10008-010-1059-z

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