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SERRS study of [Ru(CN)5(pyS)]4− SAM and cytochrome c: A suggestion toward the heterogeneous molecular recognition

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Abstract

Surface-Enhanced Raman Scattering (SERS) spectra of [Ru(CN)5(pyS)]4− (RupyS) complex self-assembled monolayer (SAM) were obtained on gold and silver surfaces at 632.8 and 413.1 nm excitation radiations, respectively. The bands assigned to the heme iron of the cytochrome c (cyt c) metalloprotein group were observed by using the RupyS SAM on silver at 413.1 nm. The Surface-Enhanced Resonance Raman Scattering (SERRS) spectra of the RupyS SAM on silver in the cyt c solution obtained at −0.2 and +0.2 V present bands at 1,365 and 1,374 cm−1 characteristic of the heme group, indicating the reduced and oxidized states of this protein, respectively. The bands observed at 1,464, 1,504, and 1,638 cm−1 are used to confirm the redox state of cyt c. The presence of the oxidized and reduced bands in function of different applied potential is an evidence that the protein is interacting with the modifier.

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References

  1. Malandrinos G, Dodi K, Louloudi M, Hadjiliadis N (2000) J Inorg Chem 79:21

    CAS  Google Scholar 

  2. Louludi M, Hadjiliadis N (1994) Coord Chem Rev 135/136:429

    Article  Google Scholar 

  3. Cotton TM, Clark RJH, Hester RE (1988) Spectroscopy of surfaces. Wiley, New York

    Google Scholar 

  4. Kneipp K, Kneipp H, Itzkan I, Dasari RR, Feld MS (1999) Chem Rev 99:2957

    Article  CAS  Google Scholar 

  5. Nie S, Emory SR (1997) Science 275:1102

    Article  CAS  Google Scholar 

  6. Diógenes ICN, de Sousa JR, de Carvalho IMM, Temperini MLA, Tanaka AA, Moreira IS (2003) Dalton Trans 11:2231

    Article  CAS  Google Scholar 

  7. Diógenes ICN, Nart FC, Temperini MLA, Moreira IS (2001) Inorg Chem 40:4884

    Article  CAS  Google Scholar 

  8. Creighton JA, Clark RJH, Hester RE (eds) (1988) Selection rules for surface-enhanced raman spectroscopy. In: Spectroscopy of surfaces. Wiley, New York

  9. Hildebrandt P, Stockburger M (1989) Biochemistry 28:6710

    Article  CAS  Google Scholar 

  10. Lu T, Yu X, Dong S, Zhou C, Ye S, Cotton TM (1994) J Electroanal Chem 369:79

    Article  CAS  Google Scholar 

  11. Hildebrandt P, Stockburger M (1986) J Phys Chem 90:6017

    Article  CAS  Google Scholar 

  12. Sousa JR, Batista AA, Diógenes ICN, Andrade GFS, Temperini MLA, Lopes LGF, Moreira IS (2003) J Electroanal Chem 543:93

    Article  Google Scholar 

  13. Sousa JR, Parente MMV, Diógenes ICN, Lopes LGF, Neto PL, Temperini MLA, Batista AA, Moreira IS (2004) J Electroanal Chem 566:443

    Article  CAS  Google Scholar 

  14. Moreira IS, Franco DW (1994) Inorg Chem 33:1607

    Article  CAS  Google Scholar 

  15. Brautigan DL, Fergusson MS, Margoliash E (1978) Methods Enzymol 53:128

    CAS  Google Scholar 

  16. Gao P, Gosztola D, Leung LWH, Weaver MJ (1987) J Electroanal Chem 233:211

    Article  CAS  Google Scholar 

  17. Albert A, Barlin GB (1959) J Chem Soc 2384

  18. Hexter RM, Albrecht MG (1979) Spectrochim Acta, Part A 35:233

    Article  Google Scholar 

  19. Cook D (1961) Can J Chem 39:2009

    Article  CAS  Google Scholar 

  20. Green JHS, Kynaston W, Paisley HM (1963) Spectrochim Acta 19:549

    Article  CAS  Google Scholar 

  21. Spinner E (1960) J Chem Soc 1237

  22. Baldwin JA, Schuhler N, Butler IS, Andrews MP (1996) Langmuir 12:6389

    Article  CAS  Google Scholar 

  23. Baldwin JA, Vlckova B, Andrews MP, Butler IS (1997) Langmuir 13:3744

    Article  CAS  Google Scholar 

  24. Yu H-Z, Xia N, Liu Z-F (1999) Anal Chem 71:1354

    Article  CAS  Google Scholar 

  25. Hu JW, Zhao B, Xu WQ, Li BF, Fan YG (2002) Spectrochim Acta, Part A 58:2827

    Article  Google Scholar 

  26. Corio P, Andrade GFS, Diógenes ICN, Moreira IS, Nart FC, Temperini MLA (2002) J Electroanal Chem 520:40

    Article  CAS  Google Scholar 

  27. Larsson S (1983) J Chem Soc, Faraday Trans 79:1375

    Article  CAS  Google Scholar 

  28. Hobara D, Niki K, Zhou C, Chumanov G, Cotton TM (1994) Colloids Surf, A Physicochem Eng Asp 93:241

    Article  CAS  Google Scholar 

  29. Gouterman M, Dolphin D (ed) (1978) The porphyrins. Academic, New York

  30. Hildebrandt P, Scott RA, Mauk AG (eds) (1996) Resonance spectroscopy of cytochrome c. University Science, Mill Valley, CA

  31. Spiro TG, Lever AB, Gray HB (eds) (1982) Iron porphyrins, Part II. Physical Bioinorganic Chemistry Series. Addison-Wesley, Reading, MA

  32. Bard AJ, Faulkner LR (1980) Electrochemical methods, fundamentals and applications. Wiley, New York

    Google Scholar 

  33. Armstrong FA (1990) Struct Bond 72:137

    Article  CAS  Google Scholar 

  34. Allen PM, Hill HAO, Walton NJ (1984) J Electroanal Chem 178:69

    Article  CAS  Google Scholar 

  35. Pinheiro SO, Silva FON, Carvalho IMM, Lopes LGF, Temperini MLA, Andrade GFS, Moreira IS, Diógenes ICN (2006) J Braz Chem Soc 17:1594

    Article  Google Scholar 

  36. Hildebrandt P, Stockburger M, Alix AJP, Bernard L, Manfait M (eds) (1985) Spectroscopy of biological molecules. Wiley, New York

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Acknowledgments

The authors I. C. N. Diógenes, I. S. Moreira, and M. L. A. Temperini would like to express their thankfulness to “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq) for grants.

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Authors and Affiliations

  1. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Cx, Postal 12200, Campus do Pici s/n, 60455-760, Fortaleza, CE, Brazil

    Izaura Cirino Nogueira Diógenes & Ícaro de Sousa Moreira

  2. Instituto de Química de São Carlos, Universidade de São Paulo (USP), Cx, Postal 369, São Carlos, SP, Brazil

    Francisco Carlos Nart

  3. Instituto de Química, Universidade de São Paulo (USP), Cidade Universitária, Cx, Postal 26077, 05599-970, São Paulo, SP, Brazil

    Mônica B. P. Mello Barreto & Marcia Laudelina Arruda Temperini

Authors
  1. Izaura Cirino Nogueira Diógenes
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  2. Francisco Carlos Nart
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  3. Mônica B. P. Mello Barreto
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  4. Marcia Laudelina Arruda Temperini
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  5. Ícaro de Sousa Moreira
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Corresponding author

Correspondence to Ícaro de Sousa Moreira.

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This paper is dedicated to Prof. Francisco Nart, in memoriam.

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Diógenes, I.C.N., Nart, F.C., Barreto, M.B.P.M. et al. SERRS study of [Ru(CN)5(pyS)]4− SAM and cytochrome c: A suggestion toward the heterogeneous molecular recognition. J Solid State Electrochem 11, 1585–1590 (2007). https://doi.org/10.1007/s10008-007-0366-5

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  • DOI: https://doi.org/10.1007/s10008-007-0366-5

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