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Adverse effects of the amlodipine on bone healing of the mandibular fracture: an experimental study in rats

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Abstract

Background

Calcium channel blockers are effective antihypertensive agents, but they may affect many metabolic processes, including bone metabolism.

Purpose

The aim of this study was to evaluate by radiographic, histologic, and biochemical analyses the effects of amlodipine on bone healing of a defect simulating a fracture in mandibular ramus of rats.

Methods

Fifty male Wistar rats were submitted to the same unilateral surgical procedure simulating a mandibular fracture. Experimental group received oral doses of amlodipine 0.04 mg/rat/day starting 12 days before procedure, while control group received water. Animals were sacrificed at 1, 7, 14, 30, and 90 days postoperatively. Numerical values were submitted to statistical analyses.

Results

Radiographic analysis showed larger radiolucent area into bone defect to the experimental group at the periods of 14 (p = 0.016), 30 (p = 0.009), and 90 (p = 0.028) days. In the histological analysis, the experimental group had a slight delay in the chronology of the repair process. In the histomorphometric analysis, the experimental group presented significant lowering of newly formed bone volume at 7 and 14 days periods (p = 0.049). There was a significant decrease of alkaline phosphatase levels in experimental group in the initial periods (p = 0.049).

Conclusions

It was concluded that chronic use of amlodipine compromised bone neoformation in the repairing process of surgical defect in the mandibular ramus of rats, but no precluded occurrence of fracture consolidation.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Maxillofacial Surgery, Prosthodontics and Traumatology, Dental School, University of São Paulo, São Paulo, São Paulo, Brazil

    Rogério Bonfante Moraes & João Gualberto Cerqueira Luz

  2. Department of Stomatology, Dental School, University of São Paulo, São Paulo, São Paulo, Brazil

    Luciana Corrêa

Authors
  1. Rogério Bonfante Moraes
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  2. Luciana Corrêa
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  3. João Gualberto Cerqueira Luz
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Correspondence to Rogério Bonfante Moraes.

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Moraes, R.B., Corrêa, L. & Luz, J.G.C. Adverse effects of the amlodipine on bone healing of the mandibular fracture: an experimental study in rats. Oral Maxillofac Surg 15, 93–101 (2011). https://doi.org/10.1007/s10006-010-0237-6

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  • DOI: https://doi.org/10.1007/s10006-010-0237-6

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