Purification of Three Spectral Isoforms of Recombinant Fluorescent Protein from Insect Bioreactors Infected with Recombinant Baculovirus
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Recombinant peptides and proteins have a wide range of applications, especially in medicine and biomedical research. Methods to rapidly and inexpensively manufacture recombinant proteins are needed to realize the full potential of proteins in medicine and to broaden their applications. A one-semester-length laboratory exercise is reported here demonstrating the use of the insect bioreactor system to create recombinant proteins. These laboratory exercises are suitable for students majoring in biochemistry or biotechnology and can be used to teach students basic biochemistry laboratory techniques including bacterial transformation, plasmid DNA isolation, insect cell culture, production of recombinant virus, use of recombinant viruses to deliver transgenes to a living organism, protein isolation by precipitation and chromatography, and protein quantification and protein characterization by electrophoresis and western blotting. The techniques acquired can also be used as the basis for related independent research projects in the second semester.
KeywordsRecombinant Protein Biomedical Research Insect Cell Full Potential Protein Isolation
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