Docking and molecular dynamics studies on triclosan derivatives binding to FabI

  • Xuyun Yang
  • Junrui Lu
  • Ming Ying
  • Jiangbei Mu
  • Peichun Li
  • Yue Liu
Original Paper


FabI, enoyl-ACP reductase (ENR), is the rate-limiting enzyme in the last step for fatty acids biosynthesis in many bacteria. Triclosan (TCL) is a commercial bactericide, and as a FabI inhibitor, it can depress the substrate (trans-2-enoyl-ACP) binding with FabI to hinder the fatty acid synthesis. The structure-activity relationship between TCL derivatives and FabI protein has already been acknowledged, however, their combination at the molecular level has never been investigated. This paper uses the computer-aided approaches, such as molecular docking, molecular dynamics simulation, and binding free energy calculation based on the molecular mechanics/Poisson-Bolzmann surface area (MM/PBSA) method to illustrate the interaction rules of TCL derivatives with FabI and guide the development of new derivatives. The consistent data of the experiment and corresponding activity demonstrates that electron-withdrawing groups on side chain are better than electron-donating groups. 2-Hydroxyl group on A ring, promoting the formation of hydrogen bond, is vital for bactericidal effect; and the substituents at 4-position of A ring, 2′-position and 4′-position of B ring benefit antibacterial activity due to forming a hydrogen bond or stabilizing the conformation of active pocket residues of receptor. While the substituents at 3′-position and 5′-position of B ring destroy the π-π stacking interaction of A ring and NAD+ which depresses the antibacterial activity. This study provides a new sight for designing novel TCL derivatives with superior antibacterial activity.


Enoyl-ACP reductase MM-PBSA Molecular dynamics simulation Triclosan 



The authors acknowledge financial support from the National Natural Science Foundation of China. (No. 21176194, 21476174)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Xuyun Yang
    • 1
  • Junrui Lu
    • 1
  • Ming Ying
    • 1
  • Jiangbei Mu
    • 1
  • Peichun Li
    • 1
  • Yue Liu
    • 1
  1. 1.Tianjin University of TechnologyTianJinChina

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