Abstract
A 3D-QSAR analysis has been carried out by comparative molecular field analysis (CoMFA) on a series of distamycin analogs that bind to the DNA of drug-resistant bacterial strains MRSA, PRSP and VSEF. The structures of the molecules were derived from the X-ray structure of distamycin bound to DNA and were aligned using the Database alignment method in Sybyl. Statistically significant CoMFA models for each activity were generated. The CoMFA contours throw light on the structure activity relationship (SAR) and help to identify novel features that can be incorporated into the distamycin framework to improve the activity. Common contours have been gleaned from the three models to construct a unified model that explains the steric and electrostatic requirements for antimicrobial activity against the three resistant strains.
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References
Clough J (2002) Drug Discov Today 7:1036–1038
Yoneyama H, Katsumata R (2006) Biotechnol Biochem 70:1060
Ford C, Hamel J, Stapert D, Moerman J, Hutchinson D, Barbachyn M, Zurenko G (1999) Infect Med 16:435–445
Andries K, Verhasselt P, Guillemont J, Göhlmann HWH, Neefs J-M, Winkler H, Gestel JV, Timmerman P, Zhu M, Lee E, Williams P, Chaffoy D, Huitric E, Hoffner S, Cambau E, Truffot-Pernot C, Lounis N, Jarlier V (2005) Science 307:223–227
Arcamone F, Penco S, Orezzi P, Nicolella V, Pirelli A (1964) Nature 203:1064–1065
Choudhary AK, Brown JR, Longmore RB (1978) J Med Chem 21:607–612
Kaizerman JA, Gross MI, Ge Y, White S, Hu W, Duan J-X, Baird EE, Johnson KW, Tanaka RD, Moser HE, Burli RW (2003) J Med Chem 46:3914–3929
Cramer RD, Patterson DE, Bunce JD (1988) J Am Chem Soc 110:5959–5967
Tanimoto TT, IBM Internal Reports 17th Nov, 1957
Sybyl 7.1, Tripos Associates Inc., 1699 S Hanley Rd, St Louis, MO 631444, USA
Halgren TJ (1990) Am Chem Soc 112:4710–4723
Bush BL, Nachbar RB (1993) J Comput-Aided Mol Des 7:587–619
Cramer RD, Bunce JD, Patterson DE, Frank IE (1988) Quant Struct-Act Relat 7:18–25
Clark M, Cramer RD, Jones DM, Patterson DE, Simeroth PE (1990) Tetrahedron Comput Methodol 3:47–59
Acknowledgements
SAK and AKM thank the Council of Scientific and Industrial Research (CSIR), India for the Fellowship. The computational facilities at BCP were provided by the All India Council for Technical Education (AICTE), India (F. No. 8022/RID/NPROJ/RPS-5/2003-04).
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Khedkar, S.A., Malde, A.K. & Coutinho, E.C. CoMFA study of distamycin analogs binding to the minor-groove of DNA: a unified model for broad-spectrum activity. J Mol Model 13, 1099–1108 (2007). https://doi.org/10.1007/s00894-007-0234-3
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DOI: https://doi.org/10.1007/s00894-007-0234-3