Abstract
Adenomatous polyposis coli (APC), a well-known anti-oncogene, is considered to have multiple functions through its several binding domains. We have continuingly studied APC1638T/1638T mice (APC1638T mice) to elucidate the functions of APC other than tumor suppression. A distinctive feature of the APC1638T mice is they are tumor free and live as long as APC+/+ mice (WT mice). Previously, we found the length of crypt–villus axis in the jejunum was significantly elongated in APC1638T mice compared with that of WT mice. The populations of goblet cells, Paneth cells, and enteroendocrine cells were also disordered in APC1638T mice. Here, we further analyzed the intestinal dyshomeostasis in APC1638T mice, focusing on the proliferation and differentiation of intestinal stem cell (ISC) lineages, and apoptotic cell shedding at the villus tips. We found that the proliferation of ISC lineages was normally controlled; however, the shedding process of apoptosis cells was significantly delayed in the APC1638T mouse jejunum. Furthermore, the number of microfold cells (M cells) was significantly increased in the APC1638T mouse jejunum. Our data suggested both differentiation process of ISCs and turnover process of intestinal epithelia were disturbed in APC1638T mice, and that contributed to the villus elongation in the APC1638T mouse jejunum.
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This work was supported by JSPS KAKENHI, Grant Number JP18K06827 to Senda T.
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All of the experiments using animals were conducted according to the regulations set by the Committee for Ethics in Animal Experimentation at Gifu University, which are in accordance with the “principles of laboratory animal care” (NIH publication no. 86-23, revised 1985).
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Wenduerma, Yamada, N.O., Wang, T. et al. A further study on a disturbance of intestinal epithelial cell population and kinetics in APC1638T mice. Med Mol Morphol 54, 203–215 (2021). https://doi.org/10.1007/s00795-020-00279-x
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DOI: https://doi.org/10.1007/s00795-020-00279-x