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Biochemical and morphological characterization of SEPT1 in mouse brain

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Abstract

Septins are a highly conserved family of GTPases which are identified in diverse organisms ranging from yeast to humans. In mammals, nervous tissues abundantly contain septins and associations of septins with neurological disorders such as Alzheimer’s disease and Parkinson’s disease have been reported. However, roles of septins in the brain development have not been fully understood. In this study, we produced a specific antibody against mouse SEPT1 and carried out biochemical and morphological characterization of SEPT1. When the expression profile of SEPT1 during mouse brain development was analyzed by western blotting, we found that SEPT1 expression began to increase after birth and the increase continued until postnatal day 22. Subcellular fractionation of mouse brain and subsequent western blot analysis revealed the distribution of SEPT1 in synaptic fractions. Immunofluorescent analyses showed the localization of SEPT1 at synapses in primary cultured mouse hippocampal neurons. We also found the distribution of SEPT1 at synapses in mouse brain by immunohistochemistry. These results suggest that SEPT1 participates in various synaptic events such as the signaling, the neurotransmitter release, and the synapse formation/maintenance.

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Acknowledgements

This work was supported in part by JSPS KAKENHI grant (grant no. 21390318) and a grant-in-aid of Takeda Science foundation. We thank Ms. Noriko Kawamura and Takako Nagano for their technical assistances.

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Authors and Affiliations

  1. Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Developmental Disability Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan

    Hidenori Ito, Rika Morishita, Mariko Noda, Ikuko Iwamoto & Koh-ichi Nagata

  2. Department of Neurochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Koh-ichi Nagata

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  1. Hidenori Ito
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  2. Rika Morishita
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  3. Mariko Noda
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  4. Ikuko Iwamoto
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  5. Koh-ichi Nagata
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Correspondence to Koh-ichi Nagata.

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Ito, H., Morishita, R., Noda, M. et al. Biochemical and morphological characterization of SEPT1 in mouse brain. Med Mol Morphol 53, 221–228 (2020). https://doi.org/10.1007/s00795-020-00248-4

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  • DOI: https://doi.org/10.1007/s00795-020-00248-4

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