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The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression

Medical Molecular Morphology volume 52, pages 187–197 (2019)Cite this article

Abstract

So far, the majority of the previous animal studies have focused on the preventive effects of resveratrol (RSV) on non-alcoholic fatty liver disease (NAFLD) rather than the therapeutic effects. In this study, the therapeutic effects of RSV on hepatic oxidative stress (OS), inflammation, and lipid metabolism-related gene expression of obese mice induced by a high-fat diet (HFD) were investigated. Male C57BL/6 mice were fed a HFD for 8 weeks to induce obesity-related NAFLD model. And then, NAFLD mice were treated with daily RSV oral gavage at the dose of 100 mg/kg body weight for an additional 4 weeks. HFD-induced the elevation of serum total cholesterol, high-density lipoprotein cholesterol, glucose, insulin, aspartate aminotransferase and alanine aminotransferase levels, and homeostasis model assessment of insulin resistance, hepatic histology changes, the increases in hepatic triglyceride, malondialdehyde and tumor necrosis factor alpha concentrations, as well as the higher mRNA expression of hepatic toll-like receptor 4 and cluster of differentiation 36 in mice, were restored by RSV. The therapeutic effects of RSV against hepatic steatosis of HFD obese mice were attributed to the reduction of OS, inflammation and free fatty acid uptake.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant nos. 31772634, 31802094 and 31601948), the National Key Research and Development Program of China (Grant no. 2018YFD0501101), the Postdoctoral Research Foundation of China (Grant no. 2018M632320), the Natural Science Foundation of Jiangsu Province (Grant no. BK20180531), and the Open Project of Shanghai Key Laboratory of Veterinary Biotechnology (Grant no. klab201710).

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Affiliations

  1. College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, China

    Kang Cheng, Zhihua Song, Hao Zhang, Simian Li, Chao Wang, Lili Zhang & Tian Wang

  2. School of electrical and Electronic Engineering, Anhui Science and Technology University, No. 1501 Mount Huangshan Avenue, Bengbu, 233100, China

    Zhihua Song

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  1. Kang Cheng
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  2. Zhihua Song
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  3. Hao Zhang
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  7. Tian Wang
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Correspondence to Tian Wang.

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Cheng, K., Song, Z., Zhang, H. et al. The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression. Med Mol Morphol 52, 187–197 (2019). https://doi.org/10.1007/s00795-019-00216-7

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Keywords

  • Resveratrol
  • Hepatic steatosis
  • Oxidative stress
  • Inflammation
  • Lipid metabolism
  • Obesity