Abstract
Platelet-rich plasma (PRP) has been established as an autologous source for therapeutic angiogenesis. The purpose of this study was to evaluate PRP angiogenic effects compared to platelet-poor plasma (PPP) in vitro and in vivo. The effects of PRP on vascular endothelial growth factor receptor-2 (VEGFR2) and CD34 expression were evaluated using real-time PCR, flow cytometry, western blot, immunocytochemistry and pathological study, as were carried out in both human umbilical endothelial cell culture and rat skin. Our findings indicated significant effect of PRP and PPP on VEGFR2 and CD34 expression by human umbilical vein endothelial cells, which was greater in latter. These effects, however, were confirmed by demonstrating an earlier angiogenic effect of PPP in vivo when compared to PRP. The findings of the present study as the first comparative study of PRP versus PPP are novel. Nevertheless, further studies are needed to clarify the underlying mechanism of these findings to improve the therapeutic effects of PRP and PPP.
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We would like to thank the Research and Enterprise Committee and the faculty of Allied Medicine, Iran University of Medical Sciences for their support.
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There is no conflict of interests that could be perceived as influencing the impartiality of the research reported and none of the authors has a financial interest in any of the products, devices, or drugs mentioned in this manuscript.
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Shahidi, M., Vatanmakanian, M., Arami, M.K. et al. A comparative study between platelet-rich plasma and platelet-poor plasma effects on angiogenesis. Med Mol Morphol 51, 21–31 (2018). https://doi.org/10.1007/s00795-017-0168-5
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DOI: https://doi.org/10.1007/s00795-017-0168-5