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Route of intrabacterial nanotransportation system for CagA in Helicobacter pylori

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Abstract

Helicobacter pylori (H. pylori) possesses an intrabacterial nanotransportation system (ibNoTS) for transporting CagA and urease within the bacterial cytoplasm; this system is controlled by the extrabacterial environment. The transportation routes of the system have not yet been studied in detail. In this study, we demonstrated by immunoelectron microscopy that CagA localizes closely with the MreB filament in the bacterium, and MreB polymerization inhibitor A22 obstructs ibNoTS for CagA. These findings indicate that the route of ibNoTS for CagA is closely associated with the MreB filament. Because these phenomena were not observed in ibNoTS for urease, the route of ibNoTS for CagA is different from that of ibNoTS for urease as previously suggested. We propose that the route of ibNoTS for CagA is associated with the MreB filament in H. pylori.

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Acknowledgments

We thank Mr. Yoshihiko Fujioka of the Department of Microbiology and Infection Control, Osaka Medical College, and Mr. Rintaro Oide of the Department of Central Research Laboratory, Osaka Medical College, for their technical help. We also thank Mrs. Hiroko Oki and Mrs. Kanako Kira for their support.

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Author notes

  1. Yukimasa Ooi

    Present address: Infection Control Office, Osaka Medical College Hospital, Osaka, Japan

Authors and Affiliations

  1. Project Team for Study of Nanotransportation System, Central Research Center, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan

    Hong Wu, Noritaka Iwai, Takashi Nakano & Kouichi Sano

  2. Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan

    Hong Wu, Takashi Nakano, Yukimasa Ooi, Sonoko Ishihara & Kouichi Sano

  3. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan

    Noritaka Iwai

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  1. Hong Wu
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Correspondence to Hong Wu.

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Wu, H., Iwai, N., Nakano, T. et al. Route of intrabacterial nanotransportation system for CagA in Helicobacter pylori . Med Mol Morphol 48, 191–203 (2015). https://doi.org/10.1007/s00795-015-0097-0

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  • DOI: https://doi.org/10.1007/s00795-015-0097-0

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