Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells

Abstract

Respiratory syncytial virus (RSV) is the major infectious agent causing serious respiratory tract inflammation in infants and young children. However, an effective vaccine and anti-viral therapy for RSV infection have not yet been developed. Hop-derived bitter acids have potent pharmacological effects on inflammation. Therefore, we investigated the effects of humulone, which is the main constituent of hop bitter acids, on the replication of RSV and release of the proinflammatory cytokine IL-8 and chemokine RANTES in RSV-infected human nasal epithelial cells (HNECs). We found that humulone prevented the expression of RSV/G-protein, formation of virus filaments and release of IL-8 and RANTES in a dose-dependent manner in RSV-infected HNECs. These findings suggest that humulone has protective effects against the replication of RSV, the virus assembly and the inflammatory responses in HNECs and that it is a useful biological product for the prevention and therapy for RSV infection.

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Acknowledgments

We thank Dr. Y. Somekawa (Sapporo Hospital of Hokkaido Railway Company) and Dr. K. Asano (KKR Sapporo Medical Center Tonan Hospital) for nasal mucosal tissues. This work was supported by the Ono Cancer Research Fund, the Suhara Memorial Foundation, the Pancreas Research Foundation of Japan, the Program for developing a supporting system for upgrading education and research, the Ministry of Education, Culture, Sports, Science, and Technology, the Ministry of Health, Labour and Welfare of Japan, and the Japan Science and Technology Agency.

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Correspondence to Takashi Kojima.

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Fuchimoto, J., Kojima, T., Okabayashi, T. et al. Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells. Med Mol Morphol 46, 203–209 (2013). https://doi.org/10.1007/s00795-013-0024-1

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Keywords

  • Humulone
  • RSV
  • Proinflammatory cytokine
  • Chemokine
  • Human nasal epithelial cells