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Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats

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Abstract

We have shown that two multidomain adaptor proteins, p140Cap and vinexin, interact with each other and are likely to be involved in neurotransmitter release. Because the basic molecular mechanism governing neurotransmitter and insulin secretion is conserved, these two proteins may also to play pivotal roles in insulin secretion. We therefore performed some characterization of p140Cap and vinexin in pancreas of a wild-type rat or a spontaneous type 2 diabetes mellitus (DM) model, the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. These two proteins were detected in Wistar rat pancreas by Western blotting. Immunohistochemistry revealed that p140Cap and vinexin are enriched in β and α cells, respectively, in the rat pancreas. We then found that pancreatic islet structure was disorganized in the OLETF rat with hyperinsulinemia or with hyperglycemia, based on immunohistochemical analyses of vinexin. In β cells of these model rats, p140Cap was distributed in a cytoplasmic granular pattern as in the control rats, although its expression was reduced to various extents from cell to cell. These results may suggest possible involvement of p140Cap in insulin secretion, and reduction of p140Cap might be related to abnormal insulin secretion in DM.

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Acknowledgments

This work was supported in part by a grant-in-aid for scientific research from Ministry of Education, Science, Technology, Sports and Culture of Japan and by a grant from Takeda Science Foundation.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of General Internal Medicine, Gifu University School of Medicine, Gifu, Japan

    Masahiro Yamauchi, Kazuo Kajita & Tatsuo Ishizuka

  2. Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, 480-0392, Japan

    Kaori Sudo, Hidenori Ito, Ikuko Iwamoto, Rika Morishita & Koh-ichi Nagata

  3. Matsunami General Hospital, Kasamatsu, Japan

    Toshihiro Murai

Authors
  1. Masahiro Yamauchi
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  2. Kaori Sudo
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  3. Hidenori Ito
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  4. Ikuko Iwamoto
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  5. Rika Morishita
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  6. Toshihiro Murai
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  7. Kazuo Kajita
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  8. Tatsuo Ishizuka
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  9. Koh-ichi Nagata
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Correspondence to Koh-ichi Nagata.

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Yamauchi, M., Sudo, K., Ito, H. et al. Localization of multidomain adaptor proteins, p140Cap and vinexin, in the pancreatic islet of a spontaneous diabetes mellitus model, Otsuka Long-Evans Tokushima Fatty rats. Med Mol Morphol 46, 41–48 (2013). https://doi.org/10.1007/s00795-013-0008-1

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  • DOI: https://doi.org/10.1007/s00795-013-0008-1

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