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Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion

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Abstract

Combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy (EM-ISH & IHC) has sufficient ultrastructural resolution to provide two-dimensional images of subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (Quantum dots; Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of the subcellular localization of pituitary hormone and its mRNA. Both EM-ISH & IHC and ISH & IHC using Qdots and CLSM are useful for understanding the relationship between protein and mRNA simultaneously in two or three dimensions. CLSM observation of rab3B and SNARE proteins such as SNAP-25 and syntaxin revealed that both rab3B and SNARE system proteins play an important role and work together as the exocytotic machinery in anterior pituitary cells. Another important issue is the intracellular transport and secretion of pituitary hormone. An experimental pituitary cell line, the GH3 cell, in which growth hormone (GH) is linked to enhanced yellow fluorescein protein (EYFP), has been developed. This stable GH3 cell secretes GH linked to EYFP upon being stimulated by Ca2+ influx or Ca2+ release from storage. This GH3 cell is useful for real-time visualization of the intracellular transport and secretion of GH. These three methods enable us to visualize consecutively the processes of transcription, translation, transport, and secretion of pituitary hormone.

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Authors and Affiliations

  1. Department of Neurosurgery, Teikyo University Chiba Medical Center, 3426-3 Anesaki, Ichihara, Chiba, 299-0111, Japan

    Akira Matsuno, Akiko Mizutani, So Yamada, Shoko M. Yamada, Hiroshi Nakaguchi, Katsumi Hoya & Mineko Murakami

  2. Teikyo Heisei University, Tokyo, Japan

    Akira Matsuno & Hiroko Okinaga

  3. Department of Nephrology and Endocrinology, University of Tokyo Hospital, Tokyo, Japan

    Koji Takano

  4. Department of Rehabilitation, Teikyo University Chiba Medical Center, Chiba, Japan

    Masato Takeuchi & Mutsumi Sugaya

  5. Teaching and Research Support Center, Tokai University School of Medicine, Kanagawa, Japan

    Johbu Itoh

  6. Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan

    Susumu Takekoshi

  7. Pathology Diagnosis Center, International University of Health and Welfare, Tokyo, Japan

    R. Yoshiyuki Osamura

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  1. Akira Matsuno
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  2. Akiko Mizutani
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  14. R. Yoshiyuki Osamura
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Correspondence to Akira Matsuno.

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Matsuno, A., Mizutani, A., Okinaga, H. et al. Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion. Med Mol Morphol 44, 63–70 (2011). https://doi.org/10.1007/s00795-011-0545-4

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