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Differential distribution of blood-derived proteins in xenografted human adenocarcinoma tissues by in vivo cryotechnique and cryobiopsy

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Abstract

Tumor behavior depends on the complex tumor interstitium and microenvironment, which influence transport of fluid and soluble molecules from blood vessels. The purpose of this study was to reveal how complex tumor tissues affect the immunodistribution of serum proteins and time-dependent translocation of bovine serum albumin (BSA) from blood vessels, using relatively differentiated human adenocarcinoma produced by the xenografted A549 cell line. Histological architecture and immunodistribution of the serum proteins in adenocarcinomatous tissues were clearly detected by the in vivo cryotechnique and cryobiopsy. Both albumin and IgG1 were detected in blood vessels, connective tissues around the tumor mass, and the interstitium among tumor cell nests. IgM was mainly detected in blood vessels and connective tissues around the tumor mass but was not detected in the interstitium among the tumor cell nests. At 10 or 30 min after BSA injection, BSA was observed only in blood vessels, but 1 h after the injection, it was also detected in the interstitium and surrounding connective tissues of the tumor mass. The present findings showed topographic variation of molecular permeation in the adenocarcinomatous tumor mass. The interstitial tissues with augmented permeability of serum proteins would increase accessibility of tumor cells to blood-derived molecules.

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Authors and Affiliations

  1. Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan

    Yuqin Bai, Nobuhiko Ohno, Nobuo Terada, Sei Saitoh & Shinichi Ohno

  2. Department of Human Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Japan

    Tadao Nakazawa, Nobuki Nakamura & Ryohei Katoh

Authors
  1. Yuqin Bai
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  2. Nobuhiko Ohno
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  3. Nobuo Terada
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  4. Sei Saitoh
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  5. Tadao Nakazawa
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  6. Nobuki Nakamura
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  7. Ryohei Katoh
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  8. Shinichi Ohno
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Corresponding author

Correspondence to Shinichi Ohno.

Additional information

Dr. Y. Bai was a research fellow from the Department of Pathology, Faculty of Medicine, Chifeng University, Inner Mongolia 024000, China, while this work was in progress at the University of Yamanashi.

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Bai, Y., Ohno, N., Terada, N. et al. Differential distribution of blood-derived proteins in xenografted human adenocarcinoma tissues by in vivo cryotechnique and cryobiopsy. Med Mol Morphol 44, 93–102 (2011). https://doi.org/10.1007/s00795-010-0512-5

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  • DOI: https://doi.org/10.1007/s00795-010-0512-5

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