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Ultrastructural study of nuclear inclusions immunohistochemically positive for surfactant protein A in pulmonary adenocarcinoma with special reference to their morphogenesis

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Abstract

To investigate the fine-structural nature of nuclear inclusions immunopositive for surfactant protein A (SP-A) antibody staining, a detailed ultrastructural study was performed, as well as immunohistochemical examination of pulmonary adenocarcinomas. Surgically resected tumor specimens from 31 patients were examined by immunohistochemistry focused on reactivity to SP-A and thyroid transcription factor 1 (TTF-1) antibodies. Only cases with >5% positive nuclear inclusions in cancer cells were considered positive, some of which were examined by electron microscopy. Immunohistochemically, 6 of 31 cases were doubly positive for SP-A and TTF-1 antibodies. On electron microscopy, SP-A-positive nuclei contained diffuse or globular fine granular substance as inclusions. Both types of globular and diffuse inclusions were sometimes connected to the inner nuclear membrane, in association with fragmented or stacked membranous structures. The findings of this study suggested that nuclear inclusions positive for SP-A antibody staining in adenocarcinomas of the lung were derived from accumulated content in the perinuclear cistern resembling pseudoinclusion processes and composed of proteins antigenically cross-reactive with SP-A.

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Authors and Affiliations

  1. Department of Pathology, Kochi Medical School, Kochi University, Kochi, Japan

    Shu-hui Lu & Mutsuo Furihata

  2. Division of Pathology, Matsuyama Shimin Hospital, Matsuyama, Ehime, 790-0067, Japan

    Yuji Ohtsuki

  3. Department of Thoracic & Cardiovascular Surgery and Regeneration Technology, Kochi University, Kochi, Japan

    Yoshiki Nonami & Shiro Sasaguri

  4. Department of Medicine and Therapeutics, Control and Prevention of Infectious Diseases, Faculty of Medicine, University of the Ryukyu, Okinawa, Japan

    Jiro Fujita

  5. Department of Surgery, Matsuyama Shimin Hospital, Matsuyama, Japan

    Masashi Uomoto

  6. Department of Pathology, Basic Faculty of Medicine, Jiamusi University, Jiamusi, China

    Fu-shan Tao

  7. Department of Hematology and Respiratory Medicine, Kochi Medical School, Kochi University, Kochi, Japan

    Makoto Kobayashi

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  1. Shu-hui Lu
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  2. Yuji Ohtsuki
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  3. Yoshiki Nonami
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Correspondence to Yuji Ohtsuki.

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Lu, Sh., Ohtsuki, Y., Nonami, Y. et al. Ultrastructural study of nuclear inclusions immunohistochemically positive for surfactant protein A in pulmonary adenocarcinoma with special reference to their morphogenesis. Med Mol Morphol 39, 214–220 (2006). https://doi.org/10.1007/s00795-006-0337-4

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  • DOI: https://doi.org/10.1007/s00795-006-0337-4

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