Abstract
The Halobacterium salinarum catalase-peroxidase gene was subcloned into shuttle vectors pWL102 and pWL202 and expressed under the control of different archaeal promoters. When Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of its own promoter, catalase-peroxidase activity increased twofold. Catalase-peroxidase activity increased threefold when Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of a tRNA promoter. This bifunctional enzyme in Hbt. salinarum was not induced by environmental stresses such as H2O2, intense light, darkness, high temperature, low temperature, redox inhibitors, heavy metals, or ions.
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Received: May 5, 2000 / Accepted: August 28, 2000
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Long, S., Salin, M. Archaeal promoter-directed expression of the Halobacterium salinarum catalase-peroxidase gene. Extremophiles 4, 351–356 (2000). https://doi.org/10.1007/s007920070004
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DOI: https://doi.org/10.1007/s007920070004