Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2AT for chitin extraction from crab and shrimp shell by-products
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The present study investigates the purification and biochemical characterization of a novel extracellular serine alkaline protease, subtilisin (called SAPN) from Melghiribacillus thermohalophilus Nari2AT. The highest yield of protease (395 IU/g) with white shrimp shell by-product (40 g/L) as a unique source of nutriments in the growth medium was achieved after 52 h at 55 °C. The monomeric enzyme of about 30 kDa was purified to homogeneity by ammonium sulfate fractionation, heat treatment, followed by sequential column chromatographies. The optimum pH and temperature values for subtilisin activity were pH 10 and 75 °C, respectively, and half lives of 9 and 5 h at 80 and 90 °C, respectively. The sequence of the 25 NH2-terminal residues pertaining of SAPN exhibited a high homology with those of Bacillus subtilisins. The inhibition by DFP and PMSF indicates that this enzyme belongs to the serine proteases family. SAPN was found to be effective in the deproteinization (DDP %) of blue swimming crab (Portunus segnis) and white shrimp (Metapenaeus monoceros) by-products, with a degree of 65 and 82%, respectively. The commercial and the two chitins obtained in this work showed a similar peak pattern in Fourier-Transform Infrared (FTIR) analysis, suggesting that SAPN is suitable for the bio-production of chitin from shell by-products.
The purification of subtilisin (SAPN) from M. thermohalophilus Nari2A was carried out.
The molecular weight and the NH2-terminal sequence of the subtilisin were determined.
Optimum pH and temperature values for activity were pH 10 and 75 °C respectively.
SAPN was found to be effective in the deproteinization of crab and shrimp by-products.
SAPN may be used as candidate for chitin extraction from crustacean by-products.
KeywordsMelghiribacillus thermohalophilus Subtilisin Portunus segnis Metapenaeus monoceros Chitin
The authors would like to express their gratitude to Mr. K. Walha, Mrs. N. Kchaou, and Mrs. N. Masmoudi (Analysis Unit-CBS) for their technical assistance. We would also like to thank Dr. W. Saibi and Pr. H. Belghith (CBS) and Mr. F. Allala (LCBM, FSB-USTHB) for their constructive discussions and suggestions. Special thanks are also due to Pr. W. Hariz from the English Department at the Faculty of Sciences of Sfax (FSS), University of Sfax (Tunisia) for constructive proofreading and language polishing services.
This study was supported by the Tunisian Ministry of Higher Education and Scientific Research under the Contract Program LMBEE-CBS/code: LR15CBS06_2015-2019 and the Ph.D. Student Fellowship of the Doctoral Institute of Fundamental Sciences of the Sfax University represented by the FSS, University of Sfax/Code: ED08FSSf01.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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