DGGE analysis combined with a metagenomic approach was used to get insights into heterotrophic anoxic enrichment cultures of four hot springs of Vale das Furnas, Portugal, using the recalcitrant substrate spent coffee ground (SCG). Parallel enrichment cultures were performed using the major components of spent coffee ground, namely arabinogalactan, galactomannan, cellulose, and proteins. DGGE revealed that heterotrophic thermophilic bacteria are highly abundant in the hydrothermal springs and significant differences in community composition depending on the substrate were observed. DNA, isolated from enrichment cultures of different locations that were grown on the same substrate were pooled, and the respective metagenomes were analyzed. Results indicated that cultures grown on recalcitrant substrate SCG consists of a totally different thermophilic community, dominated by Dictyoglomus. Enrichments with galactomannan and arabinogalactan were dominated by Thermodesulfovibrio, while cultures with casein and cellulose were dominated by Thermus. This study indicates the high potential of thermophilic bacteria degrading recalcitrant substrate such as SCG and furthermore how the accessibility to complex polymers shapes the bacterial community.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Alvarez L, Bricio C, Blesa A et al (2014) Transferable denitrification capability of Thermus thermophilus. Appl Environ Microbiol 80:19–28. https://doi.org/10.1128/AEM.02594-13
Antranikian G, Suleiman M, Schäfers C, Adams MWW, Bartolucci S, Blamey JM et al (2017) Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island. Extremophiles 21:733–742. https://doi.org/10.1007/s00792-017-0938-y
Blumer-Schuette SE, Lewis DL, Kelly RM (2010) Phylogenetic, microbiological, and glycoside hydrolase diversities within the extremely thermophilic, plant biomass-degrading genus Caldicellulosiruptor. Appl Environ Microbiol 76:8084–8092. https://doi.org/10.1128/AEM.01400-10
Campos-Vega R, Loarca-Piña G, Vergara-Castañeda HA, Oomah BD (2015) Spent coffee grounds: a review on current research and future prospects. Trends Food Sci Technol 45:24–36. https://doi.org/10.1016/j.tifs.2015.04.012
Dini-Andreote F, De Cássia Pereira E, Silva M, Triadó-Magarit X et al (2014) Dynamics of bacterial community succession in a salt marsh chronosequence: evidences for temporal niche partitioning. ISME J 8:1989–2001
Gao D, Uppugundla N, Chundawat SP et al (2011) Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides. Biotechnol Biofuels 4:5. https://doi.org/10.1186/1754-6834-4-5
He Q, Hemme CL, Jiang H et al (2011) Bioresource Technology Mechanisms of enhanced cellulosic bioethanol fermentation by co-cultivation of Clostridium and Thermoanaerobacter spp. Bioresour Technol 102:9586–9592. https://doi.org/10.1016/j.biortech.2011.07.098
Huson DH, Beier S, Flade I et al (2016) MEGAN community edition-interactive exploration and analysis of large-scale microbiome sequencing data. PLoS Comput Biol 12:1–12. https://doi.org/10.1371/journal.pcbi.1004957
Koeck DE, Pechtl A, Zverlov V, Schwarz WH (2014) Genomics of cellulolytic bacteria. Curr Opin Biotechnol 29:171–183. https://doi.org/10.1016/j.copbio.2014.07.002
Li Q, Qiao W, Wang X et al (2015) Kinetic characterization of thermophilic and mesophilic anaerobic digestion for coffee grounds and waste activated sludge. Waste Manag 36:77–85. https://doi.org/10.1016/j.wasman.2014.11.016
Liu H, Zhang T, Fang HHP (2003) Thermophilic H2 production from a cellulose-containing wastewater. Biotechnol Lett 25:365–369
Mussatto SI, Carneiro LM, Silva JPA et al (2011) A study on chemical constituents and sugars extraction from spent coffee grounds. Carbohydr Polym 83:368–374. https://doi.org/10.1016/j.carbpol.2010.07.063
Sahm K, John P, Nacke H et al (2013) High abundance of heterotrophic prokaryotes in hydrothermal springs of the Azores as revealed by a network of 16S rRNA gene-based methods. Extremophiles 17:649–662. https://doi.org/10.1007/s00792-013-0548-2
Saiki T, Kobayashi Y, Kawagoe K, Beppu T (1985) Dictyoglomus thermophilum gen. Nov., sp. nov., a chemoorganotrophic, anaerobic, thermophilic bacterium. Int J Syst Bacteriol 35:253–259
Shi R, Li Z, Ye Q et al (2013) Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum. Bioresour Technol 142:338–344. https://doi.org/10.1016/j.biortech.2013.05.037
Simões J, Nunes FM, Domingues MR, Coimbra MA (2013) Extractability and structure of spent coffee ground polysaccharides by roasting pre-treatments. Carbohydr Polym 97:81–89. https://doi.org/10.1016/j.carbpol.2013.04.067
Van Den Berg EM, Van Dongen U, Abbas B, Van Loosdrecht MCM (2015) Enrichment of DNRA bacteria in a continuous culture. ISME J 9:2153–2161. https://doi.org/10.1038/ismej.2015.26
Vishnivetskaya TA, Hamilton-Brehm SD, Podar M et al (2014) Community analysis of plant biomass-degrading microorganisms from obsidian pool, yellowstone national park. Microb Ecol 69:333–345. https://doi.org/10.1007/s00248-014-0500-8
Wanga Y, Wanga X, Tanga R et al (2010) A novel thermostable cellulase from Fervidobacterium nodosum. J Mol Catal B Enzym 66:294–301. https://doi.org/10.1016/j.molcatb.2010.06.006
Wongwilaiwalin S, Rattanachomsri U, Laothanachareon T et al (2010) Analysis of a thermophilic lignocellulose degrading microbial consortium and multi-species lignocellulolytic enzyme system. Enzyme Microb Technol 47:283–290. https://doi.org/10.1016/j.enzmictec.2010.07.013
Xu Z, Yu G, Zhang X et al (2014) The variations in soil microbial communities, enzyme activities and their relationships with soil organic matter decomposition along the northern slope of Changbai Mountain. Appl Soil Ecol 86:19–29. https://doi.org/10.1016/j.apsoil.2014.09.015
Conflict of interest
Authors declare no conflict of interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Communicated by H. Atomi.
About this article
Cite this article
Suleiman, M., Klippel, B., Busch, P. et al. Enrichment of anaerobic heterotrophic thermophiles from four Azorean hot springs revealed different community composition and genera abundances using recalcitrant substrates. Extremophiles 23, 277–281 (2019). https://doi.org/10.1007/s00792-019-01079-7
- Spent coffee ground
- Enrichment cultures
- Microbial diversity