, Volume 22, Issue 4, pp 617–628 | Cite as

Heavy metal tolerance in marine strains of Yarrowia lipolytica

  • Ashok Bankar
  • Smita Zinjarde
  • Manisha Shinde
  • Gita Gopalghare
  • Ameeta Ravikumar
Original Paper


Heavy metal tolerance of two marine strains of Yarrowia lipolytica was tested on solid yeast extract peptone dextrose agar plates. Based on minimum inhibitory concentration esteems, it is inferred that the two strains of Y. lipolytica were tolerant to heavy metals such as Pb(II), Cr(III), Zn(II), Cu(II), As(V), and Ni(II) ions. The impact of various heavy metal concentrations on the growth kinetics of Y. lipolytica was likewise assessed. With increased heavy metal concentration, the specific growth rate was reduced with delayed doubling time. Furthermore, biofilm development of both yeasts on the glass surfaces and in microtitre plates was assessed in presence of different heavy metals. In microtitre plates, a short lag phase of biofilm formation was noticed without the addition of heavy metals in yeast nitrogen base liquid media. A lag phase was extended over increasing metal concentrations of media. Heavy metals like Cr(VI), Cd(II), and As(V) are contrastingly influenced on biofilms’ formation of microtitre plates. Other heavy metals did not much influence on biofilms development. Thus, biofilm formation is a strategy of Y. lipolytica under stress of heavy metals has significance in bioremediation process for recovery of heavy metals from contaminated environment.


Yarrowia lipolytica Metal tolerance MIC Lag phase Specific growth rates 



All authors are thankful to CSIR, UGC, New Delhi, India and BCUD, Savitribai Phule Pune University, Pune, India for financial support.

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest.


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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Ashok Bankar
    • 1
    • 2
  • Smita Zinjarde
    • 1
  • Manisha Shinde
    • 2
  • Gita Gopalghare
    • 2
  • Ameeta Ravikumar
    • 1
  1. 1.Institute of Bioinformatics and BiotechnologySavitribai Phule Pune UniversityPuneIndia
  2. 2.MES Abasaheb Garware College Affiliated to Savitribai Phule Pune UniversityPuneIndia

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