Abstract
Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C50-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95–97%. The storage of biomass at 4 °C resulted in the gradual degradation of the carotenoids, which reached 58–64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.
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Abbreviations
- DMSO:
-
Dimethyl sulfoxide
- ANN:
-
Artificial neural network
- CFU:
-
Colony forming unit
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The work is financially supported with the Grant of Russian Science Foundation No. 16-19-10469.
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Communicated by A. Oren.
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Kalenov, S.V., Gordienko, M.G., Murzina, E.D. et al. Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids. Extremophiles 22, 511–523 (2018). https://doi.org/10.1007/s00792-018-1013-z
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DOI: https://doi.org/10.1007/s00792-018-1013-z
Keywords
- Halobacterium salinarum
- Spray drying
- Carotenoids
- Halophiles, artificial neural network