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Extremophiles

, Volume 22, Issue 2, pp 211–220 | Cite as

Benthic phototrophic community from Kiran soda lake, south-eastern Siberia

  • Ekaterina I. BurganskayaEmail author
  • Irina A. Bryantseva
  • Vasil A. Gaisin
  • Denis S. Grouzdev
  • Maria S. Rysina
  • Darima D. Barkhutova
  • Roman V. Baslerov
  • Vladimir M. Gorlenko
  • Boris B. Kuznetsov
Original Paper

Abstract

Phototrophic bacterial mats from Kiran soda lake (south-eastern Siberia) were studied using integrated approach including analysis of the ion composition of water, pigments composition, bacterial diversity and the vertical distribution of phototrophic microorganisms in the mats. Bacterial diversity was investigated using microscopic examination, 16S rRNA gene Illumina sequencing and culturing methods. The mats were formed as a result of decomposition of sedimented planktonic microorganisms, among which cyanobacteria of the genus Arthrospira predominated. Cyanobacteria were the largest part of phototrophs in the mats, but anoxygenic phototrophs were significant fraction. The prevailing species of the anoxygenic phototrophic bacteria are typical for soda lakes. The mats harbored aerobic anoxygenic phototrophic bacteria, purple sulfur and non-sulfur bacteria, as well as new filamentous phototrophic Chloroflexi. New strains of Thiocapsa sp. Kir-1, Ectothiorhodospira sp. Kir-2 and Kir-4, Thiorhodospira sp. Kir-3 and novel phototrophic Chloroflexi bacterium Kir15-3F were isolated and identified.

Keywords

Cyanobacterial mats Soda lakes Alkaliphilic phototrophic communities Anoxygenic phototrophic bacteria 

Notes

Acknowledgements

The authors are grateful to the workers of the Laboratory of Microbiology, Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, Ulan Ude, Russia for their help in the expedition to Kiran lake and to Samylina O.S. from Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences for her help in identification of cyanobacteria. The work was carried out using the scientific equipment of Core Research Facility “Bioengineering” with support from the Russian Foundation for Basic Research, project nos. 15-04- 07655 and 16-04- 00830, “Evolution of the organic world and planetary processes” program no. 2 of the Presidium of the Russian Academy of Sciences, Support of Scientific Schools grant of the President of the Russian Federation NSh 9888.2016.4 and project 0337-2017-0003.

Supplementary material

792_2017_989_MOESM1_ESM.pdf (2.4 mb)
Supplementary material 1 (PDF 2462 kb)

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Copyright information

© Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Ekaterina I. Burganskaya
    • 1
    Email author
  • Irina A. Bryantseva
    • 1
  • Vasil A. Gaisin
    • 1
  • Denis S. Grouzdev
    • 1
  • Maria S. Rysina
    • 2
  • Darima D. Barkhutova
    • 3
  • Roman V. Baslerov
    • 1
  • Vladimir M. Gorlenko
    • 1
  • Boris B. Kuznetsov
    • 1
  1. 1.Research Center of BiotechnologyRussian Academy of SciencesMoscowRussia
  2. 2.Moscow Institute of Physics and TechnologyMoscowRussia
  3. 3.Institute of General and Experimental BiologyRussian Academy of SciencesUlan-UdeRussia

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