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Extremophiles

, Volume 21, Issue 6, pp 1081–1090 | Cite as

Isolation and complete genome sequence of Halorientalis hydrocarbonoclasticus sp. nov., a hydrocarbon-degrading haloarchaeon

  • Dahe Zhao
  • Sumit Kumar
  • Jian Zhou
  • Rui Wang
  • Ming Li
  • Hua Xiang
Original Paper

Abstract

Bioremediation in hypersaline environments is particularly challenging since the microbes that tolerate such harsh environments and degrade pollutants are quite scarce. Haloarchaea, however, due to their inherent ability to grow at high salt concentrations, hold great promise for remediating the contaminated hypersaline sites. This study aimed to isolate and characterize novel haloarchaeal strains with potentials in hydrocarbon degradation. A haloarchaeal strain IM1011 was isolated from Changlu Tanggu saltern near Da Gang Oilfield in Tianjin (China) by enrichment culture in hypersaline medium containing hexadecane. It could degrade 57 ± 5.2% hexadecane (5 g/L) in the presence of 3.6 M NaCl at 37 °C within 24 days. To get further insights into the mechanisms of petroleum hydrocarbon degradation in haloarchaea, complete genome (3,778,989 bp) of IM1011 was sequenced. Phylogenetic analysis of 16S rRNA gene, RNA polymerase beta-subunit (rpoB’) gene and of the complete genome suggested IM1011 to be a new species in Halorientalis genus, and the name Halorientalis hydrocarbonoclasticus sp. nov., is proposed. Notably, with insights from the IM1011 genome sequence, the involvement of diverse alkane hydroxylase enzymes and an intact β-oxidation pathway in hexadecane biodegradation was predicted. This is the first hexadecane-degrading strain from Halorientalis genus, of which the genome sequence information would be helpful for further dissecting the hydrocarbon degradation by haloarchaea and for their application in bioremediation of oil-polluted hypersaline environments.

Keywords

Halophiles Haloarchaea Halorientalis Genome sequencing Hexadecane Hydrocarbon bioremediation 

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Grant no. 31330001) and the Hundred Talents Program of the Chinese Academy of Sciences (to H.X.). This work was also supported by Chinese Academy of Sciences visiting professorship for postdoctoral researchers to S.K. (Grant no. 2016PB047).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Japan KK 2017

Authors and Affiliations

  • Dahe Zhao
    • 1
    • 2
  • Sumit Kumar
    • 1
  • Jian Zhou
    • 1
  • Rui Wang
    • 1
    • 3
  • Ming Li
    • 1
  • Hua Xiang
    • 1
    • 2
  1. 1.State Key Laboratory of Microbial ResourcesInstitute of Microbiology, Chinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.College of Life SciencesUniversity of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Non-Coding RNA and Drug Discovery Key Laboratory of Sichuan ProvinceChengdu Medical CollegeChengduChina

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