Extremophiles

, Volume 21, Issue 6, pp 993–1004 | Cite as

Delignification and detoxification of peanut shell bio-waste using an extremely halophilic laccase from an Aquisalibacillus elongatus isolate

  • Rezvan Rezaie
  • Shahla Rezaei
  • Nasrin Jafari
  • Hamid Forootanfar
  • Mohammad Reza Khoshayand
  • Mohammad Ali Faramarzi
Original Paper
  • 158 Downloads

Abstract

Lignocellulose bioconversion is a harsh process requiring the use of surfactants and organic solvents. Consequently, the incorporation of laccases in this bioconversion requires the bioprospecting of enzymes that can remain stable under extreme conditions. An extracellular, highly stable laccase was produced by the halophilic isolate Aquisalibacillus elongatus in submerged liquid culture fermentation. Statistical and non-statistical strategies gave the highest enzymatic activity (8.02 U mL−1) following addition of glucose (1.7 g L−1), copper sulfate (0.8 g L−1), urea (15 g L−1), and CaCl2 (0.8 g L−1). The enzyme, after purification using a synthetic affinity support, delignified a peanut shell substrate by 45%. A pH of 8.0 and a temperature of 35 °C were optimal for delignification of this bio-waste material. Addition of [Bmim][PF6], 1,4-dioxane, acetone, and HBT promoted this bio-waste delignification. Bio-treatment in the presence of 50% [Bmim][PF6] gave a maximal lignin removal of 87%. The surfactants tested had no significant effects on the delignification yield. The laccase also detoxified the toxic phenols found in peanut shell waste. The high catalytic efficiency of this enzyme against a lignocellulosic sample under extreme conditions suggests the suitability of this laccase for industrial applications.

Keywords

Laccase Halophile Optimization Aquisalibacillus elongatus Delignification Peanut shell 

Notes

Acknowledgements

Research reported in this publication was supported by Elite Researcher Grant Committee under Award Number 943687 from the National Institutes for Medical Research Development (NIMAD), Tehran, Iran to M.A.F.

Supplementary material

792_2017_958_MOESM1_ESM.docx (175 kb)
Supplementary material 1 (DOCX 176 kb)

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Copyright information

© Springer Japan KK 2017

Authors and Affiliations

  • Rezvan Rezaie
    • 1
  • Shahla Rezaei
    • 1
  • Nasrin Jafari
    • 1
  • Hamid Forootanfar
    • 2
  • Mohammad Reza Khoshayand
    • 3
  • Mohammad Ali Faramarzi
    • 1
  1. 1.Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research CenterTehran University of Medical SciencesTehranIran
  2. 2.Department of Pharmaceutical Biotechnology, Faculty of PharmacyKerman University of Medical SciencesKermanIran
  3. 3.Department of Drug and Food Control, Faculty of PharmacyTehran University of Medical SciencesTehranIran

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