Prokaryotic assemblages in the maritime Antarctic Lake Limnopolar (Byers Peninsula, South Shetland Islands)
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The potentially metabolically active components within the prokaryotic assemblages inhabiting the Antarctic Lake Limnopolar (Byers Peninsula, Maritime Antarctica) were investigated by a polyphasic approach which included culture-dependent and culture-independent methods (based on RNA molecules). Results support previous observations on the Proteobacteria and Bacteroidetes dominance, followed by Actinobacteria, in Antarctic lakes. In particular, Alpha-, Betaproteobacteria and Bacteroidetes were mainly detected by CARD-FISH and cDNA cloning, whereas Gammaproteobacteria and Actinobacteria dominated within the cultivable fraction. Overall, this study demonstrates the survival potential and physiological heterogeneity of the prokaryotic community in the Lake Limnopolar. The microbial community composition in the lake is affected by external influences (such as marine environment by sea spray and seabird dropping, and microbial mats and mosses of the catchment). However, most external bacteria would be inactive, whereas typical polar taxa dominate the potentially active fraction and are subsidized by external nutrient sources, thus assuming the main biogeochemical roles within the lake.
KeywordsCARD-FISH Bacterial isolation cDNA clone library Antarctica Limnopolar
The authors wish to thank all the members of the LIMNOPOLAR Team for their support, as well as to the crew of the ship Las Palmas (Spanish Navy) and the Marine Technology Unit (UTM-CSIC) for logistic support. L. Michaud is particularly indebted to Mr. Hilo Moreno for his professional and enthusiastic help and support, which made sampling possible. The authors thank the reviewers for their valuable comments and suggestions on the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
This work was supported by Grants from PNRA (Programma Nazionale di Ricerche in Antartide), Italian Ministry of Education and Research (PEA 2004, Research Project PNRA 2004/1.6); MNA (Museo Nazionale dell’Antartide); Spanish Ministry of Education and Science, with one of them co-financed by the European FEDER funds (POL2006-06635 and CGL2005-06549-C02-02/ANT).
- García-Jurado F, Jiménez-Gómez F, Guerrero F (2011) Effects of a dry period on the limnological characteristics of a Mediterranean high mountain lake. Limnetica 30:5–16Google Scholar
- Kumar S, Tamura K, Nei M (2003) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 2:150–163Google Scholar
- Loy A, Lehner A, Lee N, Adamczyk J, Meier H, Ernst J, Schleifer KH, Wagner M (2002) Oligonucleotide microarray for 16S rRNA gene based detection of all recognized lineages of sulfate reducing prokaryotes in the environment. Appl Environ Microbiol 68:5064–5081CrossRefPubMedPubMedCentralGoogle Scholar
- Massana R, Murray AE, Preston CM, DeLong EF (1997) Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara channel. Appl Environ Microbiol 3:50–56Google Scholar
- Michaud L, Caruso C, Mangano S, Interdonato F, Bruni V, Lo Giudice A (2012) Predominance of Flavobacterium, Pseudomonas, and Polaromonas within the prokaryotic community of freshwater shallow lakes in the northern Victoria Land, East Antarctica. FEMS Microbiol Ecol 82:391–404CrossRefPubMedGoogle Scholar
- Neef A (1997) Application of in situ identification of bacteria to population analysis in complex microbial communities. PhD Dissertation, MunichGoogle Scholar
- Pernthaler A, Pernthaler J, Amann R (2004) Sensitive multi-color fluorescence in situ hybridization for the identification of environmental microorganisms. In: Kowalchuk G et al (eds) Molecular microbial ecology manual, 2nd edn. vol 11. Kluwer Academic Press, Dordrecht, The Netherlands, pp 711–726Google Scholar
- Stahl DA, Amann R (1991) Development and application of nucleic acid probes. In: Stackebrand E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 205–248Google Scholar
- Toro M, Camacho A, Rochera C, Rico E, Bañón M, Fernández-Valiente E, Marco E, Justel A, Avendaño MC, Ariosa Y, Vincent WF, Quesada A (2007) Limnological characteristics of the freshwater ecosystems of Byers Peninsula, Livingston Island, in maritime Antarctica. Polar Biol 30:635–649CrossRefGoogle Scholar
- Tytgat B, Verleyen E, Obbels D, Peeters K, De Wever A, D’hondt S, De Meyer T, Van Criekinge W, Vyverman W, Willems A (2014) Bacterial diversity assessment in Antarctic terrestrial and aquatic microbial mats: a comparison between bidirectional pyrosequencing and cultivation. PLoS One 9(6):e97564CrossRefPubMedPubMedCentralGoogle Scholar
- Villaescusa JA, Rochera C, Velázquez D, Rico E, Quesada A, Camacho A (2013a) Bacterioplankton summer dynamics in a maritime Antarctic Lake. Limnetica 32:253–268Google Scholar